POLR2F antibody (C-Term)

Catalog No. ABIN2667947

This Rat Monoclonal antibody specifically detects POLR2F in WB, IF, ChIP and IP. It exhibits reactivity toward Human and Mouse and has been mentioned in 3+ publications.

Quick Overview for POLR2F antibody (C-Term) (ABIN2667947)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Clone: 4F8

Product Details anti-POLR2F Antibody

Binding Specificity: C-Term

Purification: Protein G Chromatography

Immunogen: This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Antibodies in solution can be stored at -80 °C for 2 years.

Expiry Date: 6 months

References for anti-POLR2F antibody (ABIN2667947)

Catania, Pidoux, Allshire: "Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin." in: PLoS genetics, Vol. 11, Issue 3, pp. e1004986, (2015) (PubMed).

Dahiya, Liu, Nonnemacher, Dampier, Wigdahl: "CCAAT enhancer binding protein and nuclear factor of activated T cells regulate HIV-1 LTR via a novel conserved downstream site in cells of the monocyte-macrophage lineage." in: PLoS ONE, Vol. 9, Issue 2, pp. e88116, (2014) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA Pol II

Molecular Weight: 240 kDa

Gene ID: 5430

Pathways: Regulatory RNA Pathways