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Histone 3 antibody (H3K9ac)

The Mouse Monoclonal anti-Histone 3 antibody has been validated for WB, IF, ChIP, DB, ICC and ChIP-seq. It is suitable to detect Histone 3 in samples from Human and Mouse. There are 3+ publications available.
Catalog No. ABIN2668378

Quick Overview for Histone 3 antibody (H3K9ac) (ABIN2668378)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Clone

1B10
  • Binding Specificity

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    H3K9ac

    Purification

    Protein A Chromatography

    Immunogen

    This Histone H3 acetylLys9 antibody was raised against a peptide including acetyllysine 9 of human Histone H3.

    Isotype

    IgG3
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    1 μg/μL

    Buffer

    Purified IgG in 100 mM sodium phosphate pH 8.0, 90 mM trisodium citrate, 430 mM sodium chloride.
    If crystals are noted, warm to room temperature and mix gently before use.

    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Storage

    -20 °C

    Storage Comment

    Antibodies in solution can be stored at -20 °C for 2 years.

    Expiry Date

    6 months
  • Tran, Nguyen, Takabe, Yamagata, Miyake: "Pre-treatment with amitriptyline causes epigenetic up-regulation of neuroprotection-associated genes and has anti-apoptotic effects in mouse neuronal cells." in: Neurotoxicology and teratology, Vol. 62, pp. 1-12, (2017) (PubMed).

    Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

    Sanders, Hagood, Liu, Zhang, Ambalavanan, Thannickal: "Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice." in: The European respiratory journal, Vol. 43, Issue 5, pp. 1448-58, (2014) (PubMed).

  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Molecular Weight

    17 kDa

    Gene ID

    8352
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