Histone 3 antibody (H3K9me3)

Catalog No. ABIN2668398

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IF, ChIP, DB and ICC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for Histone 3 antibody (H3K9me3) (ABIN2668398)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC)

Clone: MABI 0319

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K9me3

Purification: Protein G Chromatography

Immunogen: This Histone H3 trimethylLys9 antibody was raised against a peptide containing trimethylLys9 of human Histone H3.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.45 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668398)

Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

Xiao, Shi, Fan, Zhang, Wu, Lan, Minze, Fu, Ghobrial, Liu, Li: "GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation." in: Nature communications, Vol. 6, pp. 8266, (2015) (PubMed).

Sanders, Hagood, Liu, Zhang, Ambalavanan, Thannickal: "Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice." in: The European respiratory journal, Vol. 43, Issue 5, pp. 1448-58, (2014) (PubMed).

Murphy, Cipriany, Wallin, Ju, Szeto, Hagarman, Benitez, Craighead, Soloway: "Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7772-7, (2013) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Molecular Weight: 17 kDa

Gene ID: 3020