Histone 3 antibody (H3K27me3)

Catalog No. ABIN2668416

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IF, ChIP, DB, ICC and ChIP-seq. It exhibits reactivity toward Human and Mouse and has been mentioned in 39+ publications.

Quick Overview for Histone 3 antibody (H3K27me3) (ABIN2668416)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me3

Purification: Protein A Chromatography

Immunogen: This Histone H3 trimethylLys27 antibody was raised against a peptide including trimethyllysine 27 of histone H3. testing

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668416)

Su, Wang, Lee, Stephens, Papazyan, Voronina, Krautkramer, Raman, Thorpe, Boersma, Kuznetsov, Miller, Taverna, Phillips, Denu: "Reader domain specificity and lysine demethylase-4 family function." in: Nature communications, Vol. 7, pp. 13387, (2016) (PubMed).

Matsuda, Kobayashi-Ishihara, Fujikawa, Ishida, Watanabe, Yamagishi: "Epigenetic heterogeneity in HIV-1 latency establishment." in: Scientific reports, Vol. 5, pp. 7701, (2015) (PubMed).

Lay, Liu, Kelly, Witt, Farnham, Jones, Berman: "The role of DNA methylation in directing the functional organization of the cancer epigenome." in: Genome research, Vol. 25, Issue 4, pp. 467-77, (2015) (PubMed).

Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

Harr, Luperchio, Wong, Cohen, Wheelan, Reddy: "Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type lamins." in: The Journal of cell biology, Vol. 208, Issue 1, pp. 33-52, (2015) (PubMed).

She, Baroux: "Chromatin dynamics in pollen mother cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis." in: Frontiers in plant science, Vol. 6, pp. 294, (2015) (PubMed).

Sakamoto, Akiyama, Shimada, Zhu, Yuasa, Tanaka: "DNA Methylation in the Exon 1 Region and Complex Regulation of Twist1 Expression in Gastric Cancer Cells." in: PLoS ONE, Vol. 10, Issue 12, pp. e0145630, (2015) (PubMed).

Sakashita, Kawabata, Jincho, Tajima, Kumamoto, Kobayashi, Matsui, Kono: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).

Piper, Barry, Harvey, McLeay, Smith, Harris, Mason, Stringer, Day, Wray, Gronostajski, Bailey, Boyd, Richards: "NFIB-mediated repression of the epigenetic factor Ezh2 regulates cortical development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 8, pp. 2921-30, (2014) (PubMed).

Taberlay, Statham, Kelly, Clark, Jones: "Reconfiguration of nucleosome-depleted regions at distal regulatory elements accompanies DNA methylation of enhancers and insulators in cancer." in: Genome research, Vol. 24, Issue 9, pp. 1421-32, (2014) (PubMed).

Ohno, Kanayama, Moore, Ray, Negishi: "The roles of co-chaperone CCRP/DNAJC7 in Cyp2b10 gene activation and steatosis development in mouse livers." in: PLoS ONE, Vol. 9, Issue 12, pp. e115663, (2014) (PubMed).

Bowman, Deaton, Domingues, Wang, Sadreyev, Kingston, Bender: "H3K27 modifications define segmental regulatory domains in the Drosophila bithorax complex." in: eLife, Vol. 3, pp. e02833, (2014) (PubMed).

Minkovsky, Sahakyan, Rankin-Gee, Bonora, Patel, Plath: "The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation." in: Epigenetics & chromatin, Vol. 7, pp. 12, (2014) (PubMed).

Looney, Zhang, Chen, Lee, Chari, Mao, Pelizzola, Zhang, Lister, Baker, Fernandes, Gaetz, Foshay, Clift, Zhang, Li, Vallender, Wagner, Qin, Michelini, Bugarija, Park, Aryee, Stricker, Zhou, White, Ren et al.: "Systematic mapping of occluded genes by cell fusion reveals prevalence and stability of cis-mediated silencing in somatic cells. ..." in: Genome research, Vol. 24, Issue 2, pp. 267-80, (2014) (PubMed).

Le Dily, Baù, Pohl, Vicent, Serra, Soronellas, Castellano, Wright, Ballare, Filion, Marti-Renom, Beato: "Distinct structural transitions of chromatin topological domains correlate with coordinated hormone-induced gene regulation." in: Genes & development, Vol. 28, Issue 19, pp. 2151-62, (2014) (PubMed).

Lee, Wagner, Xiao, Kim, Feng, Lazar, Moore: "Nutrient-sensing nuclear receptors coordinate autophagy." in: Nature, Vol. 516, Issue 7529, pp. 112-5, (2014) (PubMed).

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." in: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Kumar, Duester: "Retinoic acid controls body axis extension by directly repressing Fgf8 transcription." in: Development (Cambridge, England), Vol. 141, Issue 15, pp. 2972-7, (2014) (PubMed).

Kuwahara, Suzuki, Tofukuji, Yamada, Kanoh, Matsumoto, Maruyama, Kometani, Kurosaki, Ohara, Nakayama, Yamashita: "The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis." in: Nature communications, Vol. 5, pp. 3555, (2014) (PubMed).

Hu, Garruss, Gao, Morgan, Cook, Smith, Shilatifard: "The Mll2 branch of the COMPASS family regulates bivalent promoters in mouse embryonic stem cells." in: Nature structural & molecular biology, Vol. 20, Issue 9, pp. 1093-7, (2013) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Molecular Weight: 17 kDa

Gene ID: 3020