Histone 3 antibody (H3K4ac)

Catalog No. ABIN2668453

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, IF, ChIP, DB, ICC and ChIP-seq. It is suitable to detect Histone 3 in samples from Human. There are 6+ publications available.

Quick Overview for Histone 3 antibody (H3K4ac) (ABIN2668453)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K4ac

Purification: None

Immunogen: This Histone H3 acetylLys4 antibody was raised against a peptide containing acetyllysine 4 of histone H3.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668453)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Messier, Gordon, Boyd, Tye, Browne, Stein, Lian, Stein: "Histone H3 lysine 4 acetylation and methylation dynamics define breast cancer subtypes." in: Oncotarget, Vol. 7, Issue 5, pp. 5094-109, (2016) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Hagelkruys, Lagger, Krahmer, Leopoldi, Artaker, Pusch, Zezula, Weissmann, Xie, Schöfer, Schlederer, Brosch, Matthias, Selfridge, Lassmann, Knoblich, Seiser: "A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain development in the absence of its paralog." in: Development (Cambridge, England), Vol. 141, Issue 3, pp. 604-16, (2014) (PubMed).

Zhang, Lei, Lu: "Alterations of epigenetic signatures in hepatocyte nuclear factor 4? deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays." in: PLoS ONE, Vol. 9, Issue 1, pp. e84925, (2014) (PubMed).

Ma, Pan, Montgomery, Olson, Schultz: "Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 8, pp. E481-9, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Molecular Weight: 17 kDa

Gene ID: 3020