Histone 3 antibody (H3K4me3)

Catalog No. ABIN2668472

This anti-Histone 3 antibody is a Rabbit Polyclonal antibody detecting Histone 3 in WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN and CUT&Tag. Suitable for Human, Mouse and Saccharomyces cerevisiae. This Primary Antibody has been cited in 75+ publications.

Quick Overview for Histone 3 antibody (H3K4me3) (ABIN2668472)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K4me3

Purification: None

Immunogen: This Histone H3 trimethylLys4 antibody was raised against a peptide including trimethyllysine 4 of histone H3.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668472)

Henikoff, Henikoff, Kaya-Okur, Ahmad: "Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation." in: eLife, Vol. 9, (2020) (PubMed).

Brahma, Henikoff: "RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast." in: Molecular cell, Vol. 73, Issue 2, pp. 238-249.e3, (2019) (PubMed).

Pan, Guan, Liu, Li, Wang, Wu, Zhou, Zhang, Ren, Zhang, Li, Yang, Hao, Yuan, Yuan, Wang, Ju, Mao, Li, Qu, Tang, Liu: "SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2." in: Cell research, Vol. 26, Issue 2, pp. 190-205, (2016) (PubMed).

Toth, Papp, Brulois, Choi, Gao, Jung: "LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection." in: PLoS pathogens, Vol. 12, Issue 9, pp. e1005878, (2016) (PubMed).

Lowe, Gemma, Rakyan, Holland: "Sexually dimorphic gene expression emerges with embryonic genome activation and is dynamic throughout development." in: BMC genomics, Vol. 16, pp. 295, (2015) (PubMed).

Weirich, Kudithipudi, Kycia, Jeltsch: "Somatic cancer mutations in the MLL3-SET domain alter the catalytic properties of the enzyme." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 36, (2015) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Brown, Esterhazy, Sarde, London, Pullabhatla, Osma-Garcia, Al-Bader, Ortiz, Elgueta, Arno, de Rinaldis, Mucida, Lord, Noelle: "Retinoic acid is essential for Th1 cell lineage stability and prevents transition to a Th17 cell program." in: Immunity, Vol. 42, Issue 3, pp. 499-511, (2015) (PubMed).

Sabò, Kress, Pelizzola, de Pretis, Gorski, Tesi, Morelli, Bora, Doni, Verrecchia, Tonelli, Fagà, Bianchi, Ronchi, Low, Müller, Guccione, Campaner, Amati: "Selective transcriptional regulation by Myc in cellular growth control and lymphomagenesis." in: Nature, Vol. 511, Issue 7510, pp. 488-92, (2014) (PubMed).

Fereres, Simón, Mohd-Sarip, Verrijzer, Busturia: "dRYBP counteracts chromatin-dependent activation and repression of transcription." in: PLoS ONE, Vol. 9, Issue 11, pp. e113255, (2014) (PubMed).

Putiri, Tiedemann, Liu, Choi, Robertson: "Impact of human MLL/COMPASS and polycomb complexes on the DNA methylome." in: Oncotarget, Vol. 5, Issue 15, pp. 6338-52, (2014) (PubMed).

Dhimolea, Wadia, Murray, Settles, Treitman, Sonnenschein, Shioda, Soto: "Prenatal exposure to BPA alters the epigenome of the rat mammary gland and increases the propensity to neoplastic development." in: PLoS ONE, Vol. 9, Issue 7, pp. e99800, (2014) (PubMed).

Konsavage, Yochum: "The myc 3' wnt-responsive element suppresses colonic tumorigenesis." in: Molecular and cellular biology, Vol. 34, Issue 9, pp. 1659-69, (2014) (PubMed).

Asmar, Hother, Kulosman, Treppendahl, Nielsen, Ralfkiaer, Pedersen, Møller, Ralfkiaer, de Nully Brown, Grønbæk: "Diffuse large B-cell lymphoma with combined TP53 mutation and MIR34A methylation: Another "double hit" lymphoma with very poor outcome?" in: Oncotarget, Vol. 5, Issue 7, pp. 1912-25, (2014) (PubMed).

DAnselmi, Masiello, Cucina, Proietti, Dinicola, Pasqualato, Ricci, Dobrowolny, Catizone, Palombo, Bizzarri: "Microenvironment promotes tumor cell reprogramming in human breast cancer cell lines." in: PLoS ONE, Vol. 8, Issue 12, pp. e83770, (2014) (PubMed).

Kelso, Baumgart, Eickhoff, Albert, Antrecht, Lemcke, Klebl, Meisterernst: "Cyclin-dependent kinase 7 controls mRNA synthesis by affecting stability of preinitiation complexes, leading to altered gene expression, cell cycle progression, and survival of tumor cells." in: Molecular and cellular biology, Vol. 34, Issue 19, pp. 3675-88, (2014) (PubMed).

Belyea, Xu, Pentz, Medrano, Li, Hu, Turner, Legallo, Jones, Tario, Liang, Gross, Sequeira-Lopez, Gomez: "Identification of renin progenitors in the mouse bone marrow that give rise to B-cell leukaemia." in: Nature communications, Vol. 5, pp. 3273, (2014) (PubMed).

Amatori, Ballarini, Faversani, Belloni, Fusar, Bosari, Pelicci, Minucci, Fanelli: "PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples." in: Epigenetics & chromatin, Vol. 7, pp. 18, (2014) (PubMed).

Kumar, Duester: "Retinoic acid controls body axis extension by directly repressing Fgf8 transcription." in: Development (Cambridge, England), Vol. 141, Issue 15, pp. 2972-7, (2014) (PubMed).

Rafehi, Balcerczyk, Lunke, Kaspi, Ziemann, Kn, Okabe, Khurana, Ooi, Khan, Du, Chang, Haviv, Keating, Karagiannis, El-Osta: "Vascular histone deacetylation by pharmacological HDAC inhibition." in: Genome research, Vol. 24, Issue 8, pp. 1271-84, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Molecular Weight: 17 kDa

Gene ID: 3020