Leukotriene B4 Receptor/BLT antibody (Biotin)

Catalog No. ABIN2688854

This Mouse Monoclonal antibody specifically detects Leukotriene B4 Receptor/BLT in FACS. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for Leukotriene B4 Receptor/BLT antibody (Biotin) (ABIN2688854)

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Leukotriene B4 Receptor/BLT antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 203-14F11

Product Details anti-Leukotriene B4 Receptor/BLT Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 203/14F11 reacts against human leukotriene B4 receptor 1 (BLTR-1). BLTR-1 is a seven-transmembrane, G-protein-coupled receptor. The receptor plays an important role in pro-inflammatory responses. Leukotrienne B4 receptors are distributed in various hematopoietic cells including monocytes, granulocytes, and lymphocytes. Leukotriene B4 (LTB4), a product of arachidonic acid metabolism, is the ligand for BLTR-1. The immunogen used to generate 203/14F11 hybridoma was a human BLTR-1-transfected cell line. Detection of BLTR-1 expression on human peripheral monocytes and granulocytes by biotinylated 203/14F11. Human lysed whole blood were stained with 20 μL/test of biotinylated 203/14F11. Streptavidin-PE (Cat. No. 554061) was used as second-step reagent. The data reflects gating on monocytes (left panel) and granulocytes (right panel) based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated mouse IgG1 (Cat. No. 555747) as isotype control (shaded histogram).

BD Pharmingen™ Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor) - Biotin - Clone 203/14F11 - Isotype Mouse IgG1 - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human BLTR-1-transfected cell line

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Leukotriene B4 Receptor/BLT antibody (Biotin) (ABIN2688854)

Pettersson, Boketoft, Sabirsh, Nilsson, Kotarsky, Olde, Owman: "First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 520-5, (2001) (PubMed).

Dasari, Jin, Kunapuli: "Distribution of leukotriene B4 receptors in human hematopoietic cells." in: Immunopharmacology, Vol. 48, Issue 2, pp. 157-63, (2000) (PubMed).

Yokomizo, Masuda, Kato, Toda, Izumi, Shimizu: "Leukotriene B4 receptor. Cloning and intracellular signaling." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 2 Pt 2, pp. S51-5, (2000) (PubMed).

Target Details for Leukotriene B4 Receptor/BLT

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Alternative Name: BLTR-1Leukotriene B4 Receptor

Background: Synonyms: hBLTR-1