BrdU antibody

Catalog No. ABIN2688858

The Mouse Monoclonal anti-BrdU antibody has been validated for IHC (fro), FM and ICS. It is suitable to detect BrdU in samples from . There are 4+ publications available.

Quick Overview for BrdU antibody (ABIN2688858)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence Microscopy (FM), Intracellular Staining (ICS)

Clone: 3D4

Product Details anti-BrdU Antibody

Brand: BD Pharmingen™

Characteristics: Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the vells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics. The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU. Fluorescence Intensity (Linear) Profile of KG1A cells analyzed ona FACScan

BD Pharmingen™ Purified Mouse Anti- BrdU - Purified - Clone 3D4 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-BrdU antibody (ABIN2688858)

Favero, Henshaw, Grimsley-Myers, Shrestha, Beier, Dwyer: "Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain." in: The Journal of comparative neurology, Vol. 521, Issue 3, pp. 677-96, (2013) (PubMed).

Allison, Barthel, Skebo, Takechi, Kawamura, Raymond: "Ontogeny of cone photoreceptor mosaics in zebrafish." in: The Journal of comparative neurology, Vol. 518, Issue 20, pp. 4182-95, (2011) (PubMed).

Navailles, Hof, Schmauss: "Antidepressant drug-induced stimulation of mouse hippocampal neurogenesis is age-dependent and altered by early life stress." in: The Journal of comparative neurology, Vol. 509, Issue 4, pp. 372-81, (2008) (PubMed).

Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." in: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical