Integrin beta 4 antibody

Catalog No. ABIN2688876

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: Reacts with CD104, integrin β4 chain, a 205 kDa transmembrane glycoprotein which associates with CD49f (integrin α6 chain) to form the α6/β4 (CD49f/CD104) complex. CD104 is expressed on epithelial cells, Schwann cells, and some tumor cells. The CD49f/CD104 complex is located in the hemidesmosomes of the epidermis, suggesting its role in epidermal cell-basement membrane adhesion. The clone 450-9D recognizes the extracellular domain of human CD104 and is able to block the binding of clone 439-9B (another CD104 clone) to A431 cells. 450-9D is effective in flow cytometry and immunohistochemistry on frozen sections. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of A431, an epidermal carcinoma cell line, analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-Human CD104 - Purified - Clone 450-9D - Isotype Mouse IgG1, λ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 450

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Integrin beta 4 antibody (ABIN2688876)

Jones, Kurpakus, Cooper, Quaranta: "A function for the integrin alpha 6 beta 4 in the hemidesmosome." in: Cell regulation, Vol. 2, Issue 6, pp. 427-38, (1991) (PubMed).

Sonnenberg, Calafat, Janssen, Daams, van der Raaij-Helmer, Falcioni, Kennel, Aplin, Baker, Loizidou: "Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion." in: The Journal of cell biology, Vol. 113, Issue 4, pp. 907-17, (1991) (PubMed).

Kennel, Epler, Lankford, Foote, Dickas, Canamucio, Cavalierie, Cosimelli, Venturo, Falcioni: "Second generation monoclonal antibodies to the human integrin alpha 6 beta 4." in: Hybridoma, Vol. 9, Issue 3, pp. 243-55, (1990) (PubMed).

Hemler, Crouse, Sonnenberg: "Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines." in: The Journal of biological chemistry, Vol. 264, Issue 11, pp. 6529-35, (1989) (PubMed).

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104 (ITGB4 Products)

Background: Synonyms: Integrin β4 chain

Pathways: Integrin Complex