ITGAL antibody

Catalog No. ABIN2688902

This Rat Monoclonal antibody specifically detects ITGAL in FACS, IHC (fro) and BR. It exhibits reactivity toward Mouse and has been mentioned in 6+ publications.

Quick Overview for ITGAL antibody (ABIN2688902)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: 2D7

Product Details anti-ITGAL Antibody

Brand: BD Pharmingen™

Characteristics: The 2D7 antibody reacts with the 180- kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intracellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb 2D7 has been reported to block an in vitro allogeneic mixed-leukocyte reaction. The 2D7 and M17/4 (Cat. No. 553337, for the NA/LE™ format) antibodies are reported to recognize different epitopes of the CD11a molecule. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD11a - Purified - Clone 2D7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGAL antibody (ABIN2688902)

Potter, Grebe, Freiberg, Kupfer: "Formation of supramolecular activation clusters on fresh ex vivo CD8+ T cells after engagement of the T cell antigen receptor and CD8 by antigen-presenting cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 22, pp. 12624-9, (2001) (PubMed).

Kootstra, Van Der Giezen, Van Krieken, De Heer, Bruijn: "Effective treatment of experimental lupus nephritis by combined administration of anti-CD11a and anti-CD54 antibodies." in: Clinical and experimental immunology, Vol. 108, Issue 2, pp. 324-32, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Huleatt, Lefrançois: "Beta2 integrins and ICAM-1 are involved in establishment of the intestinal mucosal T cell compartment." in: Immunity, Vol. 5, Issue 3, pp. 263-73, (1996) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a

Background: Synonyms: Integrin αL chain, LFA-1α

Pathways: Activated T Cell Proliferation, Integrin Complex