CXCR4 antibody (Biotin)

Catalog No. ABIN2689044

Product Details anti-CXCR4 Antibody (Biotin)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CXCR4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 12G5 monoclonal antibody specifically binds to CD184, also known as CXCR4 and Fusin. CD184/CXCR4 is a seven-transmembrane domain, G-protein-linked, glycoprotein chemokine receptor. CD184 serves as a receptor for the C-X-C chemokine, SDF-1. It is expressed on a wide variety of hematopietic cells, vascular endothelial cells and cells of the nervous system. CD184 plays a variety of roles in hematopoiesis, vascularization and neural development. CD184 also functions as a coreceptor for infection with T-cell tropic strains of HIV-1 and as a receptor for CD4-independent infection by some HIV isolates. The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T-cell tropic isolates of HIV-1. Profile of peripheral blood lymphocytes analyzed by flow cytometry.

BD Pharmingen™ Biotin Mouse Anti-Human CD184 - Biotin - Clone 12G5 - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 12G5

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CXCR4 antibody (Biotin) (ABIN2689044)

Endres, Clapham, Marsh, Ahuja, Turner, McKnight, Thomas, Stoebenau-Haggarty, Choe, Vance, Wells, Power, Sutterwala, Doms, Landau, Hoxie: "CD4-independent infection by HIV-2 is mediated by fusin/CXCR4." in: Cell, Vol. 87, Issue 4, pp. 745-56, (1997) (PubMed).

Simmons, Wilkinson, Reeves, Dittmar, Beddows, Weber, Carnegie, Desselberger, Gray, Weiss, Clapham: "Primary, syncytium-inducing human immunodeficiency virus type 1 isolates are dual-tropic and most can use either Lestr or CCR5 as coreceptors for virus entry." in: Journal of virology, Vol. 70, Issue 12, pp. 8355-60, (1997) (PubMed).

Feng, Broder, Kennedy, Berger: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, (1996) (PubMed).

Loetscher, Geiser, OReilly, Zwahlen, Baggiolini, Moser: "Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes." in: The Journal of biological chemistry, Vol. 269, Issue 1, pp. 232-7, (1994) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184 (CXCR4 Products)

Background: Synonyms: CXCR4, Fusin, SDF-1 receptor, LAP3, LCR1, LESTR, NPYY3R, NPY3R, WHIM, HM89

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events