CXCR4 antibody (Biotin)

Catalog No. ABIN2689045

This anti-CXCR4 antibody is a Rat Monoclonal antibody detecting CXCR4 in FACS. Suitable for Mouse. This Primary Antibody has been cited in 9+ publications.

Quick Overview for CXCR4 antibody (Biotin) (ABIN2689045)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CXCR4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 2B11-CXCR4

Product Details anti-CXCR4 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 2B11/CXCR4 reacts with the mouse CD184, which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. Mouse CXCR4 showed 91 % homology at amino acid level with human CXCR4. The CXCR4 is widely expressed by hematopoietic and non hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The mouse CXCR4 gene has been mapped to chromosome 1. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR4 expression on BALB/c thymocytes by biotinylated 2B11/CXCR4. BALB/c thymocytes were stained with 0.25 μg/test of biotinylated 2B11/CXCR4 followed by Streptavidin-PE (Cat. No. 554061) and anti-mouse CD4-APC (Cat. No. 553051). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated rat IgG2b (Cat. No. 553987) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.

BD Pharmingen™ Biotin Rat Anti-Mouse CD184 - Biotin - Clone 2B11/CXCR4 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: GST-NCXCR4 fusion protein

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CXCR4 antibody (Biotin) (ABIN2689045)

Schabath, Müller, Schubel, Kremmer, Lipp, Förster: "The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 996-1004, (2000) (PubMed).

Förster, Kremmer, Schubel, Breitfeld, Kleinschmidt, Nerl, Bernhardt, Lipp: "Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1522-31, (1998) (PubMed).

Bleul, Farzan, Choe, Parolin, Clark-Lewis, Sodroski, Springer: "The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry." in: Nature, Vol. 382, Issue 6594, pp. 829-33, (1997) (PubMed).

Bleul, Wu, Hoxie, Springer, Mackay: "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 5, pp. 1925-30, (1997) (PubMed).

Heesen, Berman, Benson, Gerard, Dorf: "Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5455-60, (1997) (PubMed).

Hesselgesser, Halks-Miller, DelVecchio, Peiper, Hoxie, Kolson, Taub, Horuk: "CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons." in: Current biology : CB, Vol. 7, Issue 2, pp. 112-21, (1997) (PubMed).

Oberlin, Amara, Bachelerie, Bessia, Virelizier, Arenzana-Seisdedos, Schwartz, Heard, Clark-Lewis, Legler, Loetscher, Baggiolini, Moser: "The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1." in: Nature, Vol. 382, Issue 6594, pp. 833-5, (1997) (PubMed).

Feng, Broder, Kennedy, Berger: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, (1996) (PubMed).

Loetscher, Geiser, OReilly, Zwahlen, Baggiolini, Moser: "Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes." in: The Journal of biological chemistry, Vol. 269, Issue 1, pp. 232-7, (1994) (PubMed).

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184

Background: Synonyms: CXCR4, Fusin

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events