CCR4 antibody (Biotin)

Catalog No. ABIN2689051

This anti-CCR4 antibody is a Mouse Monoclonal antibody detecting CCR4 in FACS. Suitable for Human. This Primary Antibody has been cited in 9+ publications.

Quick Overview for CCR4 antibody (Biotin) (ABIN2689051)

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 1G1

Product Details anti-CCR4 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 1G1 reacts with CD194, also known as the human CC Chemokine Receptor type 4 (CCR4). CCR4 is expressed on activated Th2 cells, regulatory T cells, activated NK cells, basophils, monocytes and platelets. CCR4 is a seven-transmembrane, G-protein-coupled receptor, and is the specific receptor for CC chemokines, CCL22/MDC/Macrophage-Derived Chemokine and CCL17/TARC/Thymus and Activation-Regulated Chemokine. It has been reported that CCR4 mRNA is expressed mainly in the thymus and spleen. The human CCR4 gene has been mapped to chromosome 3p24. The purified form of this antibody has been reported not to be a neutralizing antibody. The immunogen used to generate the 1G1 hybridoma has been reported to be human CCR4 transfected L1.2 mouse lymphoma cells.

BD Pharmingen™ Biotin Mouse Anti-Human CD194 - Biotin - Clone 1G1 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CCR4 Transfected Cell Line

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CCR4 antibody (Biotin) (ABIN2689051)

Andrew, Ruffing, Kim, Miao, Heath, Li, Murphy, Campbell, Butcher, Wu: "C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 103-11, (2001) (PubMed).

Campbell, Haraldsen, Pan, Rottman, Qin, Ponath, Andrew, Warnke, Ruffing, Kassam, Wu, Butcher: "The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells." in: Nature, Vol. 400, Issue 6746, pp. 776-80, (1999) (PubMed).

Bonecchi, Bianchi, Bordignon, DAmbrosio, Lang, Borsatti, Sozzani, Allavena, Gray, Mantovani, Sinigaglia: "Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s." in: The Journal of experimental medicine, Vol. 187, Issue 1, pp. 129-34, (1998) (PubMed).

DAmbrosio, Iellem, Bonecchi, Mazzeo, Sozzani, Mantovani, Sinigaglia: "Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5111-5, (1998) (PubMed).

Imai, Chantry, Raport, Wood, Nishimura, Godiska, Yoshie, Gray: "Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1764-8, (1998) (PubMed).

Sallusto, Lenig, Mackay, Lanzavecchia: "Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes." in: The Journal of experimental medicine, Vol. 187, Issue 6, pp. 875-83, (1998) (PubMed).

Imai, Baba, Nishimura, Kakizaki, Takagi, Yoshie: "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 15036-42, (1997) (PubMed).

Samson, Soularue, Vassart, Parmentier: "The genes encoding the human CC-chemokine receptors CC-CKR1 to CC-CKR5 (CMKBR1-CMKBR5) are clustered in the p21.3-p24 region of chromosome 3." in: Genomics, Vol. 36, Issue 3, pp. 522-6, (1997) (PubMed).

Power, Meyer, Nemeth, Bacon, Hoogewerf, Proudfoot, Wells: "Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line." in: The Journal of biological chemistry, Vol. 270, Issue 33, pp. 19495-500, (1995) (PubMed).

Target Details for CCR4

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Alternative Name: CD194

Background: Synonyms: CCR4, C-C chemokine receptor type 4, CMKBR4, K5-5