CCR5 antibody (AA 9-30)

Catalog No. ABIN2689055

This Rat Monoclonal antibody specifically detects CCR5 in FACS. It exhibits reactivity toward Mouse and has been mentioned in 2+ publications.

Quick Overview for CCR5 antibody (AA 9-30) (ABIN2689055)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: C34

Product Details anti-CCR5 Antibody

Binding Specificity: AA 9-30

Brand: BD Pharmingen™

Characteristics: The C34-3448 antibody reacts with mouse chemokine receptor CD195 (also known as CCR5), a member of the β- chemokine receptor family. CD195 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1)α and β. In the mouse system, the gene for CD195 has been mapped to chromosome 9. CD195 mRNA is expressed in heart, spleen and liver tissues, and by macrophages and T-lymphocytes. The immunogen used to generate the C34- 3448 hybridoma was a KLH-conjugated peptide consisting of amino acids 9-30 of mouse CD195 (CCR5). Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 μg/mL, final concentration, Sigma). The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 (100 U/mL, final concentration, Cat. No. 550069). The splenocytes were stained with purified rat anti-mouse CD195 antibody (C34- 3448), followed by biotinylated mouse anti-rat IgG2c (A92-1, Cat. No. 553909) and Streptavidin- hycoerythrin (Cat. No. 554061) following BD Pharmingen's staining protocol (see Figure, Left panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control (purified A23-1, Cat. No. 553982, Right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control.

BD Pharmingen™ Purified Rat Anti-Mouse CD195 - Purified - Clone C34-3448 - Isotype Rat IgG2c, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CCR5 aa. 9-30

Isotype: IgG2c kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CCR5 antibody (ABIN2689055)

Meyer, Coyle, Proudfoot, Wells, Power: "Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14445-51, (1996) (PubMed).

Napolitano, Seamon, Leonard: "Identification of cell surface receptors for the Act-2 cytokine." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 285-9, (1990) (PubMed).

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CD195

Background: Synonyms: CCR5

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size