CCR6 antibody (Biotin)

Catalog No. ABIN2689058

This Mouse Monoclonal antibody specifically detects CCR6 in FACS. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for CCR6 antibody (Biotin) (ABIN2689058)

Target: CCR6 (Chemokine (C-C Motif) Receptor 6 (CCR6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR6 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 11A9

Product Details anti-CCR6 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 11A9 reacts with the human CC chemokine receptor, CCR6. CCR6 (previously known as BN-1, CKR-L3, DRY6, GPR-CY4 and STRL22), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokine MIP-3a/LARC/ Exodus. It has been shown that CCR6 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes, thymus, appendix. CCR6 mRNA was also detected in peripheral T- and B-lymphocytes and in CD34-derived dendritic cells. The human CCR6 gene, unlike other CCR genes, has been mapped to chromosome 6q27. The immunogen used to generate 11A9 hybridoma was KLH-conjugated N-terminus peptides of human CCR6. It does not cross-react with human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CCR8, CCR9, CXCR1, CXCR2, CXCR3, CXCR4 and CXCR5 transfectants. This antibody is NOT a neutralizing antibody. CCR6 had been clustered as CD196 in the VIIIth HLDA workshop. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of human CCR6 on peripheral lymphocytes detected by biotinylated 11A9.

BD Pharmingen™ Biotin Mouse Anti-Human CD196 (CCR6) - Biotin - Clone 11A9 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CCR6 antibody (Biotin) (ABIN2689058)

Baba, Imai, Nishimura, Kakizaki, Takagi, Hieshima, Nomiyama, Yoshie: "Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 14893-8, (1997) (PubMed).

Greaves, Wang, Dairaghi, Dieu, Saint-Vis, Franz-Bacon, Rossi, Caux, McClanahan, Gordon, Zlotnik, Schall: "CCR6, a CC chemokine receptor that interacts with macrophage inflammatory protein 3alpha and is highly expressed in human dendritic cells." in: The Journal of experimental medicine, Vol. 186, Issue 6, pp. 837-44, (1997) (PubMed).

Liao, Alderson, Su, Ullrich, Kreider, Farber: "STRL22 is a receptor for the CC chemokine MIP-3alpha." in: Biochemical and biophysical research communications, Vol. 236, Issue 1, pp. 212-7, (1997) (PubMed).

Liao, Lee, Farber: "Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27." in: Genomics, Vol. 40, Issue 1, pp. 175-80, (1997) (PubMed).

Power, Church, Meyer, Alouani, Proudfoot, Clark-Lewis, Sozzani, Mantovani, Wells: "Cloning and characterization of a specific receptor for the novel CC chemokine MIP-3alpha from lung dendritic cells." in: The Journal of experimental medicine, Vol. 186, Issue 6, pp. 825-35, (1997) (PubMed).

Zaballos, Varona, Gutiérrez, Lind, Márquez: "Molecular cloning and RNA expression of two new human chemokine receptor-like genes." in: Biochemical and biophysical research communications, Vol. 227, Issue 3, pp. 846-53, (1996) (PubMed).

Target Details for CCR6

Target: CCR6 (Chemokine (C-C Motif) Receptor 6 (CCR6))

Alternative Name: CD196 CCR6

Background: Synonyms: CCR6

Pathways: cAMP Metabolic Process