GP5 antibody

Catalog No. ABIN2689254

There are 4+ publications for this product available. The Armenian Hamster Monoclonal anti-GP5 antibody is suitable to detect GP5 in samples from Mouse and Rat. It has been validated for FACS, IP, IF and IHC (fro).

Quick Overview for GP5 antibody (ABIN2689254)

Target: GP5 (Glycoprotein V (Platelet) (GP5))

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This GP5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1C2

Product Details anti-GP5 Antibody

Characteristics: The 1C2 antibody reacts with CD42d (Platelet Glycoprotein V, GPV), a glycoprotein of 74 kDa in the mouse and 88 kDa in the rat. GPV noncovalently associates with GPIb (CD42b and c) and GPIX (CD42a) to form a receptor for von Willebrand factor on megakaryocytes and resting platelets. The binding sites for von Willebrand factor and thrombin have been localized to the GPIbachain (CD42b) of the GPIb-V-IX complex. GPV is cleaved by thrombin to release a 69- kDa soluble fragment, which contains the epitope recognized by mAb 1C2. The 1C2 antibody does not cross-react with human platelets nor with other hematopoietic cells of the mouse and rat. Anti-mouse CD41 mAb MWReg30 (Cat. No. 553848, for the FITC conjugate) may be used with mAb 1C2 to monitor mouse megakaryocyte maturation.

BD Pharmingen™ Purified Hamster Anti-Mouse and Rat CD42d - Purified - Clone 1C2 - Isotype Armenian Hamster IgG3, λ1 - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: (C57BL/6 x DBA/2)F1 mouse platelets

Isotype: IgG3 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-GP5 antibody (ABIN2689254)

Sato, Kiyokawa, Takada, Itagaki, Saito, Sekino, Suzuki, Taguchi, Mimori, Lanza, Fujimoto: "Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d)." in: Hybridoma, Vol. 19, Issue 6, pp. 455-61, (2001) (PubMed).

Sato, Kiyokawa, Taguchi, Suzuki, Sekino, Ohmi, Itagaki, Sato, Lepage, Lanza, Fujimoto: "Functional conservation of platelet glycoprotein V promoter between mouse and human megakaryocytes." in: Experimental hematology, Vol. 28, Issue 7, pp. 802-14, (2000) (PubMed).

Ravanat, Morales, Azorsa, Moog, Schuhler, Grunert, Loew, Van Dorsselaer, Cazenave, Lanza: "Gene cloning of rat and mouse platelet glycoprotein V: identification of megakaryocyte-specific promoters and demonstration of functional thrombin cleavage." in: Blood, Vol. 89, Issue 9, pp. 3253-62, (1997) (PubMed).

Takada, Saito, Kaneko, Iizuka, Kokai, Fujimoto: "Novel monoclonal antibody reactive with thrombin-sensitive 74-kDa glycoproteins present on platelets and megakaryocytes both from mouse and rat." in: Hybridoma, Vol. 14, Issue 4, pp. 361-7, (1996) (PubMed).

Target Details for GP5

Target: GP5 (Glycoprotein V (Platelet) (GP5))

Alternative Name: CD42d

Background: Synonyms: Platelet Glycoprotein V