CD55 antibody (Biotin)

Catalog No. ABIN2689338

The Mouse Monoclonal anti-CD55 antibody has been validated for FACS. It is suitable to detect CD55 in samples from Human. There are 3+ publications available.

Quick Overview for CD55 antibody (Biotin) (ABIN2689338)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: IA10

Product Details anti-CD55 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: Reacts with CD55, decay-accelerating factor (DAF), a glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein of approximately 70 kDa, expressed on hematopoietic cells. It has been suggested that the role of DAF is to protect cells from damage by autologous complement preventing the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertases, C4b2a3b and C3bBb3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of K562 cells expressing glycosylphosphatidylinositol (GPI) anchored protein analyzed on a FACScan Profile of glycosylphosphatidylinositol (GPI) achor-defective mutant K562 cell line lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD55 - Biotin - Clone IA10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD55 antibody (Biotin) (ABIN2689338)

Loveland, Szokolai, Johnstone, McKenzie: "Coordinate functions of multiple complement regulating molecules, CD46, CD55, and CD59." in: Transplantation proceedings, Vol. 26, Issue 3, pp. 1070-1, (1994) (PubMed).

Kinoshita, Medof, Silber, Nussenzweig: "Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 75-92, (1985) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: Synonyms: DAF

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity