CD6 antibody

Catalog No. ABIN2689349

This Mouse Monoclonal antibody specifically detects CD6 in FACS, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Rat and has been mentioned in 3+ publications.

Quick Overview for CD6 antibody (ABIN2689349)

Target: CD6

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX

Product Details anti-CD6 Antibody

Brand: BD Pharmingen™

Characteristics: The OX-52 antibody reacts with CD6, a 95-120- kDa antigen on T lymphocytes and thymocytes expressing αβ and γδ Tcell receptors. Rat NK cells express low levels of CD6 and are CD8a+, CD8b-, CD5-, and CD3-. In immunoprecipitation experiments, CD6 has been shown to co-precipitate with CD5 and to play a key role in CD5 tyrosine phosphorylation. Two-color evaluation of splenic T lymphocytes. LEW splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 mAb G4.18 (Cat. No. 554833) and purified mAb OX-52 (right panel), followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD6 - Purified - Clone OX-52 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: PVG-RT1c rat thoracic-duct leukocytes and splenocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD6 antibody (ABIN2689349)

Castro, Nunes, Oliveira, Tavares, Simões, Parnes, Moreira, Carmo: "OX52 is the rat homologue of CD6: evidence for an effector function in the regulation of CD5 phosphorylation." in: Journal of leukocyte biology, Vol. 73, Issue 1, pp. 183-90, (2003) (PubMed).

Lawetzky, Tiefenthaler, Kubo, Hünig: "Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta." in: European journal of immunology, Vol. 20, Issue 2, pp. 343-9, (1990) (PubMed).

Robinson, Puklavec, Mason: "MRC OX-52: a rat T-cell antigen." in: Immunology, Vol. 57, Issue 4, pp. 527-31, (1986) (PubMed).

Target Details for CD6

Target: CD6

Alternative Name: CD6

Background: Synonyms: OX-52 Antigen, Pan-T Cells