CD63 antibody

Catalog No. ABIN2689369

This anti-CD63 antibody is a Rat Monoclonal antibody detecting CD63 in FACS, IF, BI and ICS. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD63 antibody (ABIN2689369)

Target: CD63

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Clone: NVG

Product Details anti-CD63 Antibody

Brand: BD Pharmingen™

Characteristics: The NVG-2 monoclonal antibody specifically binds to CD63 which is also known as Tspan30, Melanoma 1 antigen, ME491, and C75951. CD63 is a tetraspanin which belongs to the transmembrane-4 glycoprotein superfamily (TM4SF). It constitutes a major component of lysosomal membranes and translocates to the cell surface in response to various stimuli. CD63 is expressed by activated platelets, granulocytes, monocytes, macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 can associate with other membrane molecules and functions in cellular signaling, adhesion and motility. Analyses of CD63 expression. Panel 1 and 2. Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927, Panel 1) or Purified Rat Anti-Mouse CD63 antibody (Cat. No. 564221, Panel 2). The cells were washed and counterstained with PE Goat Anti-Rat Ig (Cat. No. 550767). After washing, the cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 553129/561083). Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 (or Ig Isotype control staining) for gated events with the forward and sid light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Panel 3. Immunofluorescent staining of CD63 in bEnd.3 cells. Adherent bEnd.3 cells (ATCC CRL-2299) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 (Sigma, X100), and stained with either Purified Rat IgG2a, κ Isotype Control (Upper Panel 3) or Purified Rat Anti-Mouse CD63 antibody (Lower Panel 3). The cells were washed and then counterstained with Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) Antibody (Life Technologies, Cat. No. A11006, pseudocolored green) and Hoechst 33342 Solution (Cat. No. 561908, pseudocolored blue). Images were analyzed using a BD Pathway™ 435 cell imager and merged using BD AttoVision™ Software.

BD Pharmingen™ Purified Rat Anti-Mouse CD63 - Purified - Clone NVG-2 - Isotype Rat IgG2a, κ - Reactivity Ms - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Eosinophils

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD63 antibody (ABIN2689369)

Verjan Garcia, Umemoto, Saito, Yamasaki, Hata, Matozaki, Murakami, Jung, Woo, Seoh, Jang, Aozasa, Miyasaka: "SIRP?/CD172a regulates eosinophil homeostasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2268-77, (2011) (PubMed).

Miyamoto, Homma, Hotta: "Molecular cloning of the murine homologue of CD63/ME491 and detection of its strong expression in the kidney and activated macrophages." in: Biochimica et biophysica acta, Vol. 1217, Issue 3, pp. 312-6, (1994) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: Synonyms: Tspan30, Melanoma 1 antigen, ME491, C75951