CD63 antibody

Catalog No. ABIN2689370

This Mouse Monoclonal antibody specifically detects CD63 in FACS, IHC and IP. It exhibits reactivity toward Rat and has been mentioned in 2+ publications.

Quick Overview for CD63 antibody (ABIN2689370)

Target: CD63

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: AD1

Product Details anti-CD63 Antibody

Brand: BD Pharmingen™

Characteristics: The AD1 antibody reacts with a 50-60- kDa protein that is a homolog (87 % ) of human CD63 (melanomaassociated antigen ME491). CD63, a highly glycosylated type-III lysosomal glycoprotein, is a member of the tetraspan transmembrane 4 superfamily (TM4SF) that also includes CD9, CD37, and CD53. In the rat, CD63 is expressed on mast cells and platelets. CD63 is also found on rat embryonic fibroblasts and hepatic cell, along with cultured cell lines of diverse origin (eg, liver, lung, and heart). Expression is upregulated with culture. CD63 is expressed in close proximity to the FcεRI on rat mast cells and has been shown to partially inhibit IgE-mediated histamine release. The function of CD63 has not been fully characterized. The expression of CD63 on rat CD54+ peritoneal lavage cells. Lewis rat peritoneal lavage cells were incubated simultaneously with either purified Mouse IgG1, κ isotype control (Cat. no. 557273, open histogram) or purified AD1 mAb (shaded histogram), followed by biotinylated A85-1 mAb (rat anti-mouse IgG1, Cat. no. 553441) and finally Streptavidin-FITC (Cat. no. 554060) and PE-conjugated 1A29 mAb (anti-rat CD54, Cat. no. 554970). For analysis, a gate was drawn to include CD54+ cells only (gate not shown). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD63 - Purified - Clone AD1 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat basophilic leukemia cell line

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD63 antibody (ABIN2689370)

Boros, Takaichi, Masuda, Newlands, Hatanaka: "Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat." in: Shock (Augusta, Ga.), Vol. 3, Issue 2, pp. 125-31, (1995) (PubMed).

Nishikata, Oliver, Mergenhagen, Siraganian: "The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 862-70, (1992) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: Synonyms: ME491