CD73 antibody

Catalog No. ABIN2689391

This Rat Monoclonal antibody specifically detects CD73 in FACS, IHC (p), IHC (fro) and IHC (zinc). It exhibits reactivity toward Mouse and has been mentioned in 2+ publications.

Quick Overview for CD73 antibody (ABIN2689391)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: TY-23

Product Details anti-CD73 Antibody

Brand: BD Pharmingen™

Characteristics: The TY/23 antibody reacts with CD73 or Ecto-5'-nucleotidase (5'-NT), a 69 kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. CD73 expression appears to be developmentally regulated on leukocytes. In the bone marrow, it is found on most CD11b+ myeloid cells and very few CD19+ cells of the B-lymphocyte lineage. It is not found on CD11b+ cells in the periphery nor on marrow-derived GMCSF-stimulated dendritic cells. Some peripheral B lymphocytes express CD73, with higher levels on isotype-switched B cells. The few thymocytes which have detectable surface CD73 are of the CD4-CD8- (double negative) and the single-positive populations. In the peripheral lymphoid organs, significant subpopulations of the CD4+ and CD8+ T lymphocytes express CD73, with variation in the percentages of CD73-bearing T cells observed among inbred mouse strains. In the thymus and peripheral lymphoid organs, CD73 is found on endothelia and stromal cells. CD73 has also been detected on bone marrow and thymic epithelial cell lines, kidney glomeruli and proximal-tubule epithelial cells, and liver endothelial cells and hepatocytes. The TY/23 mAb inhibits the enzymatic activity of CD73. Although soluble TY/23 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with PMA, but not with Concanavalin-A or plate-bound anti-CD3ε mAb 145-2C11. Two-color analysis of the expression of CD73 on splenic T and B lymphocytes. C57BL/6 splenocytes were stained with either purified isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified mAb TY/23 (middle and right panels) followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-PE (Cat. No. 554061). T or B lymphocytes were identified by staining with FITC-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553061/553062, left and middle panels) or FITC-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557398/553785, right panel), respectively. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD73 - Purified - Clone TY/23 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse splenocytes and CHO cells transfected with the Mouse CD73 gene, Nt5

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD73 antibody (ABIN2689391)

Yamashita, Hooker, Jiang, Laurent, Resta, Khare, Coe, Kincade, Thompson: "CD73 expression and fyn-dependent signaling on murine lymphocytes." in: European journal of immunology, Vol. 28, Issue 10, pp. 2981-90, (1998) (PubMed).

Resta, Yamashita, Thompson: "Ecto-enzyme and signaling functions of lymphocyte CD73." in: Immunological reviews, Vol. 161, pp. 95-109, (1998) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: Synonyms: Ecto-5'-nucleotidase

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process