CD90 antibody (Biotin)

Catalog No. ABIN2689450

This anti-CD90 antibody is a Mouse Monoclonal antibody detecting CD90 in FACS. Suitable for Mouse, Rat, Guinea Pig and Rabbit. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD90 antibody (Biotin) (ABIN2689450)

Target: CD90 (THY1)

Reactivity: Mouse, Rat, Guinea Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: OX

Product Details anti-CD90 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: CD90 (Thy-1) is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. The OX-7 clone reacts with rat CD90 reported to be expressed by hematopoietic stem cells, early myeloid & erythroid cells, immature B lymphocytes in the bone marrow & peripheral lymphoid organs, thymocytes, recent thymic emigrants (a subset of CD45RC- peripheral T lymphocytes), neurons, glomerular mesangial cells, endothelium at inflammatory sites, mast cells, and dendritic cells. Rat dendritic epidermal T cells (DEC) have been reported to be CD90 (Thy-1) negative, unlike those of the mouse. The OX-7 clone has been reported to crossreact with the mouse CD90.1 (Thy-1.1) alloantigen of the AKR/J and PL strains, but not CD90.2 (Thy-1.2) found on many mouse strains. In the mouse, CD90 is found on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), hematopoietic stem cells, and neurons, but not B lymphocytes. In addition, there is evidence that CD90 mediates adhesion of mouse thymocytes to mouse thymic stroma. The OX-7 clone has also been reported to crossreact with rabbit and guinea pig thymus, brain, and intestine. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD90 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with biotin mouse anti-rat CD90/mouse CD90.1 (clone OX-7) (right panel) and PE mouse anti-rat CD3 (clone G4.18) (Cat. No. 554833) monoclonal antibodies, followed by avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan ™ instrument.

BD Pharmingen™ Biotin Mouse Anti-Rat CD90/Mouse CD90.1 - Biotin - Clone OX-7 (also known as OX7) - Isotype Mouse IgG1, κ - Reactivity Rat, Ms, G Pig, Rab - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat thymocyte Thy-1 antigen

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD90 antibody (Biotin) (ABIN2689450)

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, (1991) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90.1

Background: Synonyms: Rat Thy-1, Mouse Thy-1.1

Pathways: Cell-Cell Junction Organization