CXCR5 antibody

Catalog No. ABIN2689482

The Rat Monoclonal anti-CXCR5 antibody has been validated for FACS. It is suitable to detect CXCR5 in samples from Human. There are 5+ publications available.

Quick Overview for CXCR5 antibody (ABIN2689482)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CXCR5 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: RF8B2

Product Details anti-CXCR5 Antibody

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody RF8B2 reacts with the human CXC chemokine receptor, CXCR5. CXCR5 (aka BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells suggested that this receptor might function as a regulator of B cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on human peripheral lymphocytes by purified RF8B2. Human peripheral blood mononuclear cells were stained with 0.25 μg/test of purified RF8B2 using 3-step staining protocol outlined above and anti-human CD3-FITC (Cat. No. 555339, left panel) or anti-human CD19 FITC (Cat. No. 555412, right panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2b (Cat. No. 555846 and FITC-conjugated mouse IgG2a (Cat. No. 555573) or FITC-conjugated mouse IgG1 (Cat. No. 555748) as isotype controls. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed using a BD FACScan™ Flow Cytometer

BD Pharmingen™ Purifed Rat Anti-Human CXCR5 - Purified - Clone RF8B2 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CXCR5

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CXCR5 antibody (ABIN2689482)

Gunn, Ngo, Ansel, Ekland, Cyster, Williams: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).

Legler, Loetscher, Roos, Clark-Lewis, Baggiolini, Moser: "B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5." in: The Journal of experimental medicine, Vol. 187, Issue 4, pp. 655-60, (1998) (PubMed).

Förster, Emrich, Kremmer, Lipp: "Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells." in: Blood, Vol. 84, Issue 3, pp. 830-40, (1994) (PubMed).

Kouba, Vanetti, Wang, Schäfer, Höllt: "Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue." in: FEBS letters, Vol. 321, Issue 2-3, pp. 173-8, (1993) (PubMed).

Dobner, Wolf, Emrich, Lipp: "Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma." in: European journal of immunology, Vol. 22, Issue 11, pp. 2795-9, (1992) (PubMed).

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CXCR5