Cytochrome C antibody

Catalog No. ABIN2689486

This anti-Cytochrome C antibody is a Mouse Monoclonal antibody detecting Cytochrome C in WB, IP and BI. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 4+ publications.

Quick Overview for Cytochrome C antibody (ABIN2689486)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), BioImaging (BI)

Clone: 6H2-B4

Product Details anti-Cytochrome C Antibody

Brand: BD Pharmingen™

Characteristics: A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c and d) according to spectrochemical characteristics, and there are five cytochromes between coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble protein that either promotes cell survival or death, depending upon its intracellular location. In healthy cells, it is a peripheral membrane protein of the mitochondria that transports electrons from the coenzyme QH2 cytochrome c reductase complex to the cytochrome c oxidase complex. When proapoptotic stimuli induce breakdown of the mitochondria, cytochrome c is released to the cytosol where it functions in the activation of caspases that trigger apoptosis. The 6H2.B4 monoclonal antibody has been reported to recognize the native and not the denatured form of rat, mouse, and human cytochrome c. Furthermore, studies utilizing competitive ELISA indicate that mAb 6H2.B4 binds to a region around residue 62 of rat cytochrome c. The 6H2.B4 monoclonal antibody is not useful for western blot analysis. For the western blot application, clone 7H8.2C12 (cat.no. 556433) would be recommended. Suggested positive controls for detecting cytochrome c include P388D mouse lymphoma cells (ATCC CCL-46), HeLa human carcinoma cells (ATCC CCL-2), Jurkat T leukemia cells (ATCC TIB-152) and NIH 3T3 mouse fibroblast cells (ATCC CRL-1658). This antibody is routinely tested by immunoprecipitation. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Cytochrome c - Purified - Clone 6H2.B4 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Cytochrome c

Isotype: IgG1 kappa

Application Details

Application Notes: Confirmed by immunoprecipitation: Human Confirmed during development by immunoprecipitation: Mouse, Rat

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Cytochrome C antibody (ABIN2689486)

Garrido, Galluzzi, Brunet, Puig, Didelot, Kroemer: "Mechanisms of cytochrome c release from mitochondria." in: Cell death and differentiation, Vol. 13, Issue 9, pp. 1423-33, (2006) (PubMed).

Rossé, Olivier, Monney, Rager, Conus, Fellay, Jansen, Borner: "Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c." in: Nature, Vol. 391, Issue 6666, pp. 496-9, (1998) (PubMed).

Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, (1996) (PubMed).

Jemmerson, Johnson: "Different functional boundaries for the major antigenic region of two cytochromes c." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4428-32, (1991) (PubMed).

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome C

Molecular Weight: 15 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity