FOXP1 antibody

Catalog No. ABIN2689508

This Mouse Monoclonal antibody specifically detects FOXP1 in WB, IHC (fro), IHC (f) and ICS. It exhibits reactivity toward Human and Mouse and has been mentioned in 10+ publications.

Quick Overview for FOXP1 antibody (ABIN2689508)

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Clone: JC12

Product Details anti-FOXP1 Antibody

Brand: BD Pharmingen™

Characteristics: The JC12 monoclonal antibody specifically binds to Forkhead box protein P1 (FoxP1). FoxP1 belongs to subfamily P of the forkhead box (FOX) winged-helix transcription factor family. It plays a variety of roles such as regulating the formation of lymphoid, lung, brain and heart tissues. FoxP1 is critical in regulating early B cell development and is also required for maintenance of naive T cell quiescence, and monocyte differentiation and macrophage function. Aberrant expression of FOXP1 has been linked with mucosa-associated lymphoid tissue lymphoma and diffuse-large B cell lymphoma. Panel 1. Western blot analysis of FoxP1 expression in human Ramos cell lysate. Lysate prepared from cells of the human Ramos (Burkitt's lymphoma, ATCC CRL-1596) cell line was electrophoresed (SDS-PAGE), transferred to a polyvinylidene difluoride membrane, and Western blotted using Purified Anti-FoxP1 antibody (Cat. No. 564213, 2 μg/mL, overnight). Human Forkhead box protein P1 was identified as a band whose size ranged from 75-85 kDa. Panel 2. Immunohistochemical staining of acetone-fixed human tonsil for FoxP1. Acetone-fixed, frozen human tonsil sections were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 554126) or Purified Mouse Anti-FoxP1 antibody. A three-step staining procedure using Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin-HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The FoxP1-specific antibody primarily stained some lymphocytes and epithelial cells with a nuclear pattern. Original magnification: 20X. Panel 3. Immunohistochemical staining of formalin-fixed human tonsil for FoxP1. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG2a κ Isotype Control or Purified Mouse Anti-Human FoxP1 antibody. A three-step staining procedure was similarly used as before to develop the primary staining reagents. The FoxP1-specific antibody primarily stained some lymphocytes with a nuclear pattern. Original magnification: 40X.

BD Pharmingen™ Purified Mouse Anti-FoxP1 - Purified - Clone JC12 - Isotype Mouse IgG2a, κ - Reactivity Ms, Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-FOXP1 antibody (ABIN2689508)

Sagardoy, Martinez-Ferrandis, Roa, Bunting, Aznar, Elemento, Shaknovich, Fontán, Fresquet, Perez-Roger, Robles, De Smedt, Sagaert, Melnick, Martinez-Climent: "Downregulation of FOXP1 is required during germinal center B-cell function." in: Blood, Vol. 121, Issue 21, pp. 4311-20, (2013) (PubMed).

Katoh, Igarashi, Fukuda, Nakagama, Katoh: "Cancer genetics and genomics of human FOX family genes." in: Cancer letters, Vol. 328, Issue 2, pp. 198-206, (2012) (PubMed).

Feng, Wang, Takata, Day, Willen, Hu: "Transcription factor Foxp1 exerts essential cell-intrinsic regulation of the quiescence of naive T cells." in: Nature immunology, Vol. 12, Issue 6, pp. 544-50, (2011) (PubMed).

Brown, Ashe, Leich, Burek, Barrans, Fenton, Jack, Pulford, Rosenwald, Banham: "Potentially oncogenic B-cell activation-induced smaller isoforms of FOXP1 are highly expressed in the activated B cell-like subtype of DLBCL." in: Blood, Vol. 111, Issue 5, pp. 2816-24, (2008) (PubMed).

Shi, Sakuma, Mooroka, Liscoe, Gao, Croce, Sharma, Kaplan, Greaves, Wang, Simon: "Down-regulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function." in: Blood, Vol. 112, Issue 12, pp. 4699-711, (2008) (PubMed).

Hu, Wang, Borde, Nardone, Maika, Allred, Tucker, Rao: "Foxp1 is an essential transcriptional regulator of B cell development." in: Nature immunology, Vol. 7, Issue 8, pp. 819-26, (2006) (PubMed).

Streubel, Vinatzer, Lamprecht, Raderer, Chott: "T(3;14)(p14.1;q32) involving IGH and FOXP1 is a novel recurrent chromosomal aberration in MALT lymphoma." in: Leukemia, Vol. 19, Issue 4, pp. 652-8, (2005) (PubMed).

Wlodarska, Veyt, De Paepe, Vandenberghe, Nooijen, Theate, Michaux, Sagaert, Marynen, Hagemeijer, De Wolf-Peeters: "FOXP1, a gene highly expressed in a subset of diffuse large B-cell lymphoma, is recurrently targeted by genomic aberrations." in: Leukemia, Vol. 19, Issue 8, pp. 1299-305, (2005) (PubMed).

Wang, Lin, Li, Tucker: "Multiple domains define the expression and regulatory properties of Foxp1 forkhead transcriptional repressors." in: The Journal of biological chemistry, Vol. 278, Issue 27, pp. 24259-68, (2003) (PubMed).

Banham, Beasley, Campo, Fernandez, Fidler, Gatter, Jones, Mason, Prime, Trougouboff, Wood, Cordell: "The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p." in: Cancer research, Vol. 61, Issue 24, pp. 8820-9, (2001) (PubMed).

Target Details for FOXP1

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Alternative Name: FoxP1

Background: Synonyms: Forkhead box protein P1, hFKH1B, HSPC215, QRF1, 12CC4

Molecular Weight: 75-85 kDa

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response