FOXP3 antibody

Catalog No. ABIN2689510

Product Details anti-FOXP3 Antibody

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Baboon, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: The 259D/C7 antibody reacts with the human FoxP3 transcription factor, a member of the forkhead or winged helix family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ regulatory T cells and represents a specific marker for these cells. Flow-cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of CD4+CD25int cell population are FoxP3 positive. Approximately 5-10 % of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this hypothesis, it has been found that FOXP3 is mutated in scurfy (sf) mice. The 259D/C7 antibody reacts with all currently identified isoforms of human FoxP3 and is cross-reactive with Cynomolgus, Rhesus and Baboon. Flow cytometric analysis of Purified anti-Human FoxP3 on resting PBMC. Human PBMC were fixed and permeabilized using working solutions of FoxP3 Buffers A and C (Cat. No. 560098. see Recommended Assay Procedure), Intracellular staining with Purified Mouse anti-Human FoxP3 (clone 259D/C7, Cat. No. 560044) was followed by secondary staining with APC Rat anti-Mouse IgG1 (

BD Pharmingen™ Purified Mouse anti-Human FoxP3 - Purified - Clone 259D/C7 - Isotype Mouse IgG1 - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: FoxP3 recombinant protein

Clone: 259D-C7

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-FOXP3 antibody (ABIN2689510)

Roncador, Brown, Maestre, Hue, Martínez-Torrecuadrada, Ling, Pratap, Toms, Fox, Cerundolo, Powrie, Banham: "Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level." in: European journal of immunology, Vol. 35, Issue 6, pp. 1681-91, (2005) (PubMed).

Brunkow, Jeffery, Hjerrild, Paeper, Clark, Yasayko, Wilkinson, Galas, Ziegler, Ramsdell: "Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse." in: Nature genetics, Vol. 27, Issue 1, pp. 68-73, (2001) (PubMed).

Wildin, Ramsdell, Peake, Faravelli, Casanova, Buist, Levy-Lahad, Mazzella, Goulet, Perroni, Bricarelli, Byrne, McEuen, Proll, Appleby, Brunkow: "X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome is the human equivalent of mouse scurfy." in: Nature genetics, Vol. 27, Issue 1, pp. 18-20, (2001) (PubMed).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FoxP3 (FOXP3 Products)

Background: Synonyms: Scurfin, IPEX, JM2

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation