MHC, Class I antibody (Biotin)

Catalog No. ABIN2689544

This Mouse Monoclonal antibody specifically detects MHC, Class I in FACS, ELISA and IHC (fro). It exhibits reactivity toward Mouse and has been mentioned in 4+ publications.

Quick Overview for MHC, Class I antibody (Biotin) (ABIN2689544)

Target: MHC, Class I

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SF1

Product Details anti-MHC, Class I Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The SF1-1.1 antibody reacts with the α3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g, b, j, k, p, q, s, v) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e, Cat. No. 557306/553058). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[d] - Biotin - Clone SF1-1.1 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse cells

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-MHC, Class I antibody (Biotin) (ABIN2689544)

Noun, Reboul, Abastado, Jaulin, Kourilsky, Pla: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, (1996) (PubMed).

Zhao, Iwata: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." in: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).

Abastado, Casrouge, Kourilsky: "Differential role of conserved and polymorphic residues of the binding groove of MHC class I molecules in the selection of peptides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3569-75, (1993) (PubMed).

Matsumoto, Setoyama, Umesaki: "Differential induction of major histocompatibility complex molecules on mouse intestine by bacterial colonization." in: Gastroenterology, Vol. 103, Issue 6, pp. 1777-82, (1993) (PubMed).

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: H-2K[d]