Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)
Brand
BD Pharmingen™
Characteristics
The R8-294 monoclonal antibody specifically binds to Neurogenic differentiation factor 1 (NeuroD1). NeuroD1 is a basic helix-loop-helix (bHLH) transcription factor that is also known as BHLHA3, Beta-cell E-box transactivator 2, BETA2 and NeuroD. NeuroD1 interacts with other bHLH proteins and binds the insulin E-box sequence to regulate pancreatic islet cell development. It also plays a key role in central nervous system (CNS) and sensory nervous system neuron development. Specifically, it is required for the terminal differentiation of neurons during late stages of neurogenesis in the CNS (cerebral cortex, hippocampus, and cerebellum) and the sensory nervous system (eye, inner ear, and olfactory system). During antibody development, the purified R8-294 monoclonal antibody was found to detect NeuroD1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis. Western Blot analysis of NeuroD1 in human Retinoblastoma cell lysate. Lysate from Y79 cells (ATCC, HTB-18™) was probed with Purified Mouse Anti-NeuroD1 monoclonal antibody at titrations of 0.125 (lane 1), 0.06 (lane 2), and 0.03 μg/mL (lane 3). NeuroD1 is identified as a band of approximately 45 kDa. Immunoflourescent staining of NeuroD1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 Buffer, and stained with Purified Mouse Anti-NeuroD1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
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