Substance P antibody

Catalog No. ABIN2689911

The Rat Monoclonal anti-Substance P antibody has been validated for IHC (f). It is suitable to detect Substance P in samples from Rat and Pig. There are 4+ publications available.

Quick Overview for Substance P antibody (ABIN2689911)

Target: Substance P

Reactivity: Rat, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This Substance P antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NC1-34

Product Details anti-Substance P Antibody

Brand: BD Pharmingen™

Characteristics: Peptides with transmitter-like characteristics are found in many areas of the brain, and may cause inhibition, excitation or both when applied to target neurons. These peptides are synthesized as secretory products and grouped into families according structural and functional similarity. Substance P, a member of the tachykinin family, is thought to play a role in nociceptive transmission in the dorsal horn of the spinal cord. Its proposed role is based on a number of anatomical and physiological findings. For example, substance P is present in high concentration in a subpopulation of fine-diameter primary sensory axons and in nerve terminals in dorsal horn regions where primary sensory nociceptors terminate. It specifically excites nociceptive dorsal horn and is released in the spinal cord in vivo during activation of nociceptive primary sensory fibers. Additionally, substance P receptor antagonists have been shown to block nociceptive responses in vivo and in vivo. NC1/34 is specific for substance P. It reacts with mammalian substance P and has been shown to also cross-react with a substance P-like protein in the pigeon brain. It binds to subpopulations of nerve terminals and cell bodies in the central nervous system. The initial characterization of the antibody was in rat brain. The antibody does not cross-react with other mammalian brain peptides tested including [Leu]enkephalin, [Met]enkephalin, somatostatin, or β-endorphin. NC1/34 recognizes an epitope in the carboxy terminal region of substance P and thus does not differentiate substance P from neurokinins A and B. Substance P conjugated to carrier protein was used as immunogen. Paraformaldehyde-fixed, frozen tissue section of rat cerebellum stained for Substance P (mAb NC1/34) using a DAB Chromogen and Hematoxylin counterstain. Arrows indicate staining of nerve fibers.

BD Pharmingen™ Purified Rat Anti-Substance P - Purified - Clone NC1/34 - Isotype Rat IgG2a - Reactivity Rat, Pg - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Substance P conjugated to carrier protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Substance P antibody (ABIN2689911)

Ruscheweyh, Forsthuber, Schoffnegger, Sandkühler: "Modification of classical neurochemical markers in identified primary afferent neurons with Abeta-, Adelta-, and C-fibers after chronic constriction injury in mice." in: The Journal of comparative neurology, Vol. 502, Issue 2, pp. 325-36, (2007) (PubMed).

Cuello, Priestley, Milstein: "Immunocytochemistry with internally labeled monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 2, pp. 665-9, (1982) (PubMed).

Del Fiacco, Cuello: "Substance P- and enkephalin-containing neurones in the rat trigeminal system." in: Neuroscience, Vol. 5, Issue 4, pp. 803-15, (1980) (PubMed).

Cuello, Galfre, Milstein: "Detection of substance P in the central nervous system by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, Issue 7, pp. 3532-6, (1979) (PubMed).

Target Details for Substance P

Target: Substance P

Molecular Weight: 15 kDa