SDC4 antibody

Catalog No. ABIN2689914

The Rat Monoclonal anti-SDC4 antibody has been validated for ELISA and FACS. It is suitable to detect SDC4 in samples from Mouse. There are 4+ publications available.

Quick Overview for SDC4 antibody (ABIN2689914)

Target: SDC4 (Syndecan 4 (SDC4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SDC4 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: KY-8-2

Product Details anti-SDC4 Antibody

Brand: BD Pharmingen™

Characteristics: The KY/8.2 mAb reacts with mouse syndecan-4, a cell-surface heparan sulfate proteoglycan. Although the ligand(s) for this molecule is unknown, syndecans are known to interact with the heparin-binding domain of growth factors, chemokines, and extracellular matrix components. Accordingly, heparin inhibits the binding of syndecan-4 to the pre-B cell line BC7.12. Syndecan-4 is localized to focal adhesions and binds protein-kinase Cα. Thus, it is involved in cytoskeletal organization and signaling. Syndecan-4 is expressed on stromal cells and B-lymphocyte precursors in bone marrow, in addition to most splenic B cells and a small population of T lymphocytes. It is highly expressed on activated B lymphocytes, but is downregulated in isotype-switched memory B cells. The ectodomain of syndecan-4 is shed from cell surfaces constitutively and in response to specific physiological signals, and the resulting soluble fragments are functional. Immobilized KY/8.2 mAb induced the formation of dendritic processes on syndecan-4-transfected cells and on anti-CD40-activated splenic B cells. Other methods of activating B cells have not produced the same effect. This antibody recognizes an epitope in the N-terminus of syndecan-4, regardless of post-translational modifications.

BD Pharmingen™ Purified Rat Anti-Mouse Syndecan-4 - Purified - Clone KY/8.2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BCB10 mouse pre-B cell line

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SDC4 antibody (ABIN2689914)

Woods, Couchman: "Syndecan-4 and focal adhesion function." in: Current opinion in cell biology, Vol. 13, Issue 5, pp. 578-83, (2001) (PubMed).

Fitzgerald, Wang, Park, Murphy, Bernfield: "Shedding of syndecan-1 and -4 ectodomains is regulated by multiple signaling pathways and mediated by a TIMP-3-sensitive metalloproteinase." in: The Journal of cell biology, Vol. 148, Issue 4, pp. 811-24, (2000) (PubMed).

Yamashita, Oritani, Miyoshi, Wall, Bernfield, Kincade: "Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5940-8, (1999) (PubMed).

Rapraeger, Ott: "Molecular interactions of the syndecan core proteins." in: Current opinion in cell biology, Vol. 10, Issue 5, pp. 620-8, (1998) (PubMed).

Target Details for SDC4

Target: SDC4 (Syndecan 4 (SDC4))

Alternative Name: Syndecan-4

Pathways: Glycosaminoglycan Metabolic Process