TCR beta antibody (Biotin)

Catalog No. ABIN2689918

This anti-TCR beta antibody is a Armenian Hamster Monoclonal antibody detecting TCR beta in FACS and IHC (fro). Suitable for Mouse. This Primary Antibody has been cited in 7+ publications.

Quick Overview for TCR beta antibody (Biotin) (ABIN2689918)

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This TCR beta antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: H57

Product Details anti-TCR beta Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes and peripheral T lymphocytes and NK1.1+ thymocytes and NK-T cells of all mouse strains tested. It does not react with γδTCR-bearing T cells. In the fetal and adult thymus, the TCR βchain may form homodimers or pair with the pre-TCR achain on the surface of immature thymocytes before expression of the TCR achain. Plate-bound or soluble H57-597 antibody activates αβTCR-bearing T cells, and plate-bound mAb can induce apoptotic death. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. αβ TCR expression in spleen and thymus. BALB/c splenocytes (left panels) were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553048/553049), PE-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553032/553033), and either biotinylated Hamster IgG2, κ isotype control mAb Ha4/8 (Cat. no. 553963, top left panel) or biotinylated mAb H57-597 (bottom left panel) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). BALB/c thymocytes (right panels) were stained with either biotinylated isotype control (top right panel) or biotinylated mAb H57-597 (bottom right panel), followed by Avidin-FITC. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse TCR β Chain - Biotin - Clone H57-597 - Isotype Armenian Hamster IgG2, λ1 - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10

Isotype: IgG2 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TCR beta antibody (Biotin) (ABIN2689918)

Vicari, Zlotnik: "Mouse NK1.1+ T cells: a new family of T cells." in: Immunology today, Vol. 17, Issue 2, pp. 71-6, (1996) (PubMed).

Bendelac, Killeen, Littman, Schwartz: "A subset of CD4+ thymocytes selected by MHC class I molecules." in: Science (New York, N.Y.), Vol. 263, Issue 5154, pp. 1774-8, (1994) (PubMed).

Saint-Ruf, Ungewiss, Groettrup, Bruno, Fehling, von Boehmer: "Analysis and expression of a cloned pre-T cell receptor gene." in: Science (New York, N.Y.), Vol. 266, Issue 5188, pp. 1208-12, (1994) (PubMed).

Groettrup, von Boehmer: "T cell receptor beta chain dimers on immature thymocytes from normal mice." in: European journal of immunology, Vol. 23, Issue 6, pp. 1393-6, (1993) (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).

Gascoigne: "Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3." in: The Journal of biological chemistry, Vol. 265, Issue 16, pp. 9296-301, (1990) (PubMed).

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

Target Details for TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Alternative Name: TCR beta Chain