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Actin antibody (pan)

Name: Actin antibody (pan)
SKU: ABIN271986
Price: 630 USD
Availability: InStock

Reactivity: Human, Rat, Mouse WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated

Image no. 2 for anti-Actin (pan) antibody (ABIN271986) (Actin antibody (pan))

Image no. 1 for anti-Actin (pan) antibody (ABIN271986) (Actin antibody (pan))

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Catalog No. ABIN271986

Datasheet as PDF

Target See all Actin Antibodies

Actin
Binding Specificity
16

15

13

13

11

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
pan
Reactivity
98

77

73

24

21

20

12

11

6

5

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Human, Rat, Mouse
Host
84

40

3

1

1
Rabbit
Compatible Secondaries
Clonality
76

53
Polyclonal
Conjugate
73

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This Actin antibody is un-conjugated
Application
100

36

30

29

28

27

23

18

17

15

10

3

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity

This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Cross-Reactivity (Details)

Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification

Affinity-chromatography

Purity

> 95 % (by SDS-PAGE)

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Reactivity: Human, Rat, Mouse, Rabbit, Dog WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin antibody
Reactivity: Human, Rat, Mouse, Chicken, Dog, Cow, Pig, Guinea Pig WB, ELISA, IHC (p), IF (cc), IF (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Actin (C-Term) antibody
Reactivity: Human, Rat, Mouse WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Actin antibody
Reactivity: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 367-377) antibody
Reactivity: Human, Rat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 21-70) antibody
Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 189-218) antibody
Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 38-67), (N-Term) antibody
Reactivity: Human, Mouse WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated

anti-Actin (C-Term) antibody
Reactivity: Human IHC (p), IHC (fro) Host: Rabbit Monoclonal S12-I unconjugated

Application Notes

ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1,0 mg/mL

Buffer

PBS, pH 7.2, 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

Actin

Abstract

Actin Products

Background

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Molecular Weight

approx. 45 kDa