Actin antibody (pan)

Catalog No. ABIN271986

This anti-Actin antibody is a Rabbit Polyclonal antibody detecting Actin in WB, IF and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for Actin antibody (pan) (ABIN271986)

Target: Actin

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Actin Antibody

Binding Specificity: pan

Specificity: This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity-chromatography

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Actin

Target: Actin

Background: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Molecular Weight: approx. 45 kDa