NFIA antibody

Catalog No. ABIN272219

The Rabbit Polyclonal anti-NFIA antibody has been validated for WB and EIA. It is suitable to detect NFIA in samples from Human, Mouse and Rat.

Quick Overview for NFIA antibody (ABIN272219)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NFIA Antibody

Specificity: This antibody detects endogenous levels of NF-1 protein. (region surrounding Gln34)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA

Background: NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.Synonyms: CCAAT-box-binding transcription factor, CTG, NF-I/A, NFI-A, Nuclear factor 1 A-type, TGGCA-binding protein

Molecular Weight: approx. 55 kDa

Gene ID: 4774

NCBI Accession: NP_001128145

UniProt: Q12857