CDH15 antibody

Catalog No. ABIN272294

The Rabbit Polyclonal anti-CDH15 antibody has been validated for WB and EIA. It is suitable to detect CDH15 in samples from Human, Mouse and Rat.

Quick Overview for CDH15 antibody (ABIN272294)

Target: CDH15 (M-Cadherin (CDH15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDH15 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CDH15 Antibody

Specificity: This antibody detects endogenous levels of M-cadherin protein. (region surrounding Ala108)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CDH15

Target: CDH15 (M-Cadherin (CDH15))

Alternative Name: Cadherin-15

Background: Cadherins are a multigene family of Ca++-dependent cell adhesion molecules. They are transmembrane glycoproteins consisting of an extracellular domain, which mediates Ca++-dependent intercellular adhesion by homophilic interactions, a transmembrane region and a cytoplasmic domain. The extracellular domain is divided into a series of subdomains designated EC1-EC5. Homolgies between different members of the cadherin family are most prominent in the cytoplasmic domain and in EC1 and EC2 and much less so in EC5 of the extracellular domain and in the transmembrane region. The binding properties and specificities of the adhesive function are located in the N-terminal part of the molecules. Four members of the cadherin family have been identified and molecularly cloned from mammalian cells. These include the neuronal (N), epithelial (E), placental (P) and muscle (M) cadherins. M-cadherin is not found in fibroblasts but is expressed at low level in myoblasts and is upregulated following induction of myotube formation, suggesting a specific function in skeletal muscle cell differentiation.Synonyms: CDH14, CDH15, CDH3, Cadherin M, Cadherin-14, M-Cadherin, Muscle cadherin

Molecular Weight: approx. 91 kDa

Gene ID: 1013

NCBI Accession: NP_004924

UniProt: P55291