CD3 epsilon antibody (Biotin)

Catalog No. ABIN2739337

This anti-CD3 epsilon antibody is a Hamster Monoclonal antibody detecting CD3 epsilon in FACS, WB, IP and ELISA. Suitable for Mouse.

Quick Overview for CD3 epsilon antibody (Biotin) (ABIN2739337)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody (Biotin)

Specificity: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

Purification: Protein G purified

Immunogen: Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated 5 minutes with 20 μL of 250 μg/mL Mouse IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.34 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated with 20^l of 0.5 mg/mL unlabeled anti-CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gene ID: 916

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway