CD3 epsilon antibody (FITC)

Catalog No. ABIN2739339

The Hamster Monoclonal anti-CD3 epsilon antibody has been validated for FACS, WB, IP and ELISA. It is suitable to detect CD3 epsilon in samples from Mouse.

Quick Overview for CD3 epsilon antibody (FITC) (ABIN2739339)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody (FITC)

Specificity: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

Purification: Protein G purified

Immunogen: Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/FITC at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C

Storage Comment: Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gene ID: 916

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway