CD3 epsilon antibody
Quick Overview for CD3 epsilon antibody (ABIN2739340)
Target
See all CD3 epsilon (CD3E) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.
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Purification
- Protein G purified
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Immunogen
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Mouse BM10-37 T cell membrane prep.
Type of Immunogen: Membrane preparation -
Isotype
- IgG
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Application Notes
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Approved: ELISA, Flo, IP, WB
Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD3 antibody diluted to 10 μg/mL. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33 % sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.
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Preservative
- Gentamicin sulfate
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Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store at 4°C, avoid freeze-thaw cycles.
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- CD3 epsilon (CD3E)
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Alternative Name
- CD3E
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Background
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Name/Gene ID: CD3E
Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon -
Gene ID
- 916
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Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
Target
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