CD79a antibody (AA 202-216)

Catalog No. ABIN2739648

The Mouse Monoclonal anti-CD79a antibody is suitable to detect CD79a in samples from Human. It has been validated for FACS, IF, WB, IHC, ELISA, IHC (p) and IP.

Quick Overview for CD79a antibody (AA 202-216) (ABIN2739648)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: JCB117

Product Details anti-CD79a Antibody

Binding Specificity: AA 202-216

Specificity: Human CD79A / CD79 Alpha

Purification: Protein G purified

Immunogen: Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for this CD79a antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Baboon, Monkey (93%), Bat, Pig (87%), Galago, Bovine (80%), Mouse (73%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B-cell Ag receptor. CD79a first appears at pre B-cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. It is found in the majority of acute leukemias of B-cell precursors, lines and lymphomas, and in some myelomas. It is not present in myeloid or Tcell lines. CD79a antibody is generally used to complement CD20 mAb, especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This CD79a antibody will stain many of the same lymphomas as CD20 mAb, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 mAb. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: CD79A

Background: Name/Gene ID: CD79A

Synonyms: CD79A, CD79a antigen, Ig-alpha, MB-1, MB-1 membrane glycoprotein, Surface IgM-associated protein, MB1

Gene ID: 973

Pathways: BCR Signaling