GFAP antibody (AA 417-432)

Catalog No. ABIN2741090

This Rabbit Polyclonal antibody specifically detects GFAP in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Rabbit, Monkey, Horse and Gibbon.

Quick Overview for GFAP antibody (AA 417-432) (ABIN2741090)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Rabbit, Monkey, Horse, Gibbon

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFAP Antibody

Binding Specificity: AA 417-432

Specificity: Human GFAP

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP (417-432aa DGEVIKESKQEHKDVM). Percent identity by BLAST analysis: Human, Gibbon, Galago, Marmoset, Rat, Cat, Rabbit, Horse (100%), Pig, Dog (94%), Mouse (88%), Opossum (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (0.5 - 1 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Glial fibrillary acidic protein is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. Expression is also seen in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. The protein has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2.5 % BSA, 0.025 % sodium azide/thimerosal.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Name/Gene ID: GFAP

Synonyms: GFAP

Gene ID: 2670