BMF antibody (AA 13-27, AA 112-126)

Catalog No. ABIN2745519

The Rat Monoclonal anti-BMF antibody has been validated for WB, IHC, ELISA, IP, FACS and ICC. It is suitable to detect BMF in samples from Mouse and Rat.

Quick Overview for BMF antibody (AA 13-27, AA 112-126) (ABIN2745519)

Target: BMF (Bcl2 Modifying Factor (BMF))

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This BMF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 17A9

Product Details anti-BMF Antibody

Binding Specificity: AA 13-27, AA 112-126

Specificity: Recognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25 kDa and ~30 kDa by Western blot, corresponding to endogenous Bmf and BmfL.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

No Cross-Reactivity: Human

Cross-Reactivity (Details): Does not cross-react with human Bmf.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 and aa 112-126 of mouse Bmf.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for BMF

Target: BMF (Bcl2 Modifying Factor (BMF))

Alternative Name: Bmf

Background: Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis.

UniProt: Q91ZE9