VASP antibody

Catalog No. ABIN2745575

This Rabbit Polyclonal antibody specifically detects VASP in WB, IP and ICC. It exhibits reactivity toward Human, Mouse and Pig.

Quick Overview for VASP antibody (ABIN2745575)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Human, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-VASP Antibody

Specificity: Recognizes both the 46 kDa (Ser157 dephospho-) and 50 kDa (Ser157 phospho-) form of human, mouse and porcine VASP.

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine)

Purification: Antigen affinity purified.

Components: Contains Positive Control: Human platelet protein (500μg) (Prod. No. AG-35T-0001), supplied at 5mg/ml in SDS-stop solution (100mM NaCl, 73mM Tris/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% [v/v] glycerol, 2% [w/v] SDS, 10μg/ml bromophenol blue sodium salt). Use 5μl (25μg) per lane.

Immunogen: Recombinant human VASP fused to a His-tag.

Application Details

Application Notes: Use 5 μL (25 μg) per lane.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 1 mg/mL BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP

Background: VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation