CD79a antibody (AA 208-222)

Catalog No. ABIN2749074

The Mouse Monoclonal anti-CD79a antibody has been validated for FACS, WB, IHC (p) and IP. It is suitable to detect CD79a in samples from Human, Mouse, Rat, Cow, Pig, Chicken, Rabbit, Horse, Guinea Pig, Dog and Non-Human Primate. There are 7+ publications available.

Quick Overview for CD79a antibody (AA 208-222) (ABIN2749074)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reactivity: Human, Mouse, Rat, Cow, Pig, Chicken, Rabbit, Horse, Guinea Pig, Dog, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: HM47

Product Details anti-CD79a Antibody

Binding Specificity: AA 208-222

Purpose: Anti-Hu CD79a Purified

Specificity: The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Cross-Reactivity (Details): Human, Non-Human Primates, Porcine, Mouse, Rat, Bovine, Canine (Dog), Equine (Horse), Guinea pig, Rabbit, Chicken

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD79a antibody (ABIN2749074)

Zhao, Hassan, Perry, Ning, Stass, Dehner: "C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology." in: International journal of clinical and experimental pathology, Vol. 1, Issue 1, pp. 65-74, (2008) (PubMed).

Bhargava, Kallakury, Ross, Azumi, Bagg: "CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner." in: American journal of clinical pathology, Vol. 128, Issue 2, pp. 306-13, (2007) (PubMed).

Fernandez, West, Jackson, Kidney: "Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors." in: Veterinary pathology, Vol. 42, Issue 4, pp. 437-45, (2005) (PubMed).

Islas-Ohlmayer, Padgett-Thomas, Domiati-Saad, Melkus, Cravens, Martin, Netto, Garcia: "Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus." in: Journal of virology, Vol. 78, Issue 24, pp. 13891-900, (2004) (PubMed).

Torlakovic, Torlakovic: "B-cell markers in lymphocyte predominance Hodgkin disease." in: Archives of pathology & laboratory medicine, Vol. 126, Issue 7, pp. 862-3, (2002) (PubMed).

Rassenti, Kipps: "Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion." in: Blood, Vol. 95, Issue 8, pp. 2725-7, (2000) (PubMed).

Mason, Cordell, Tse, van Dongen, van Noesel, Micklem, Pulford, Valensi, Comans-Bitter, Borst: "The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 2474-82, (1991) (PubMed).

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: CD79a

Background: CD79a molecule,CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.,BCR alpha, Ig-alpha, MB-1, IGA

Gene ID: 973

UniProt: P11912

Pathways: BCR Signaling