TCR alpha/beta antibody (PE)

Catalog No. ABIN2749096

This anti-TCR alpha/beta antibody is a Mouse Monoclonal antibody detecting TCR alpha/beta in FACS. Suitable for Rat and Non-Human Primate. This Primary Antibody has been cited in 11+ publications.

Quick Overview for TCR alpha/beta antibody (PE) (ABIN2749096)

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Reactivity: Rat, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR alpha/beta antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: R73

Product Details anti-TCR alpha/beta Antibody (PE)

Purpose: Anti-Rt TCR alpha/beta PE

Specificity: The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Cross-Reactivity (Details): Non-Human Primates, Rat

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Rat T blasts and erythrocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-TCR alpha/beta antibody (PE) (ABIN2749096)

Fuller, Bogdani, Tupling, Jensen, Pefley, Manavi, Cort, Blankenhorn, Mordes, Lernmark, Kwitek: "Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene." in: Physiological genomics, Vol. 38, Issue 1, pp. 89-97, (2009) (PubMed).

Müller, van den Brandt, Odoardi, Tischner, Herath, Flügel, Reichardt: "A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats." in: The Journal of clinical investigation, Vol. 118, Issue 4, pp. 1405-16, (2008) (PubMed).

Radojevic, Arsenovic-Ranin, Kosec, Pesic, Pilipovic, Perisic, Plecas-Solarovic, Leposavic: "Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level." in: The Journal of endocrinology, Vol. 192, Issue 3, pp. 669-82, (2007) (PubMed).

Shao, Sun, Kaplan, Sun: "Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2849-54, (2004) (PubMed).

Kerstan, Hünig: "Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 3, pp. 1341-5, (2004) (PubMed).

Li, Thanikachalam, Pang, Kawaharada, Aitouche, Pham: "A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts." in: The Annals of thoracic surgery, Vol. 72, Issue 4, pp. 1306-10, (2001) (PubMed).

Ritter, Schröder, Risch, Vergopoulos, Shean, Kolls, Brock, Lehmann, Volk: "Ischemia/reperfusion injury-mediated down-regulation of adenovirus-mediated gene expression in a rat heart transplantation model is inhibited by co-application of a TNFRp55-Ig chimeric construct." in: Gene therapy, Vol. 7, Issue 14, pp. 1238-43, (2000) (PubMed).

Carlson, Jansson, Larsson, Bucht, Lorentzen: "The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats." in: The American journal of pathology, Vol. 156, Issue 6, pp. 2057-65, (2000) (PubMed).

Wilhelm, Pratschke, Beato, Taal, Kusaka, Hancock, Tilney: "Activation of the heart by donor brain death accelerates acute rejection after transplantation." in: Circulation, Vol. 102, Issue 19, pp. 2426-33, (2000) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." in: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Hünig, Wallny, Hartley, Lawetzky, Tiefenthaler: "A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes." in: The Journal of experimental medicine, Vol. 169, Issue 1, pp. 73-86, (1989) (PubMed).

Target Details for TCR alpha/beta

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Alternative Name: TCR alpha/beta

Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.,TCRA/B