LY9 antibody

Catalog No. ABIN2749218

The Mouse Monoclonal anti-LY9 antibody has been validated for WB, FACS, IP, ICC and Func. It is suitable to detect LY9 in samples from Human. There is 1 publication available.

Quick Overview for LY9 antibody (ABIN2749218)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY9 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Clone: HLy9-25

Product Details anti-LY9 Antibody

Purpose: Anti-Hu CD229 Purified Low Endotoxin

Specificity: The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: CD299-transfected 300.19 pre-B cell line

Isotype: IgG1

Application Details

Application Notes: Functional application: Regulation of activation,
Flow cytometry: Recommended dilution: 6-10 μg/mL, positive control: peripheral blood lymphocytes.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-LY9 antibody (ABIN2749218)

de la Fuente, Tovar, Villamor, Zapater, Pizcueta, Campo, Bosch, Engel: "Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9." in: Blood, Vol. 97, Issue 11, pp. 3513-20, (2001) (PubMed).

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229

Background: Lymphocyte antigen 9,CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.,SLAMF3, LY9

Gene ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling