Topoisomerase I antibody

Catalog No. ABIN2763824

The Rabbit Polyclonal anti-Topoisomerase I antibody has been validated for IHC (p). It is suitable to detect Topoisomerase I in samples from Human.

Quick Overview for Topoisomerase I antibody (ABIN2763824)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Topoisomerase I Antibody

Cross-Reactivity: Human

Characteristics: DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Purification: Antigen Immunoaffinity Purification

Immunogen: Synthetic peptide made to human DNA Topoisomerase 1

Isotype: Ig Fraction

Application Details

Application Notes: Antibody can be used for immunohistochemistry at 5-10 μg/mL on paraffin-embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: DNA Topoisomerase 1

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance