ACLY antibody (Middle Region)

Catalog No. ABIN2773810

This Rabbit Polyclonal antibody specifically detects ACLY in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Rabbit, Zebrafish (Danio rerio), Sheep, Dog, Horse and Guinea Pigand has been mentioned in 1 publication.

Quick Overview for ACLY antibody (Middle Region) (ABIN2773810)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human, Mouse, Rat, Pig, Cow, Rabbit, Zebrafish (Danio rerio), Sheep, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACLY antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACLY Antibody

Binding Specificity: Middle Region

Sequence: SRTASFSESR ADEVAPAKKA KPAMPQDSVP SPRSLQGKST TLFSRHTKAI

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 86%, Horse: 92%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 100%, Rat: 93%, Sheep: 92%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ACLY. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ACLY

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1101 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACLY antibody (ABIN2773810)

Convertini, Menga, Andria, Scala, Santarsiero, Castiglione Morelli, Iacobazzi, Infantino: "The contribution of the citrate pathway to oxidative stress in Down syndrome." in: Immunology, Vol. 149, Issue 4, pp. 423-431, (2016) (PubMed).

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ACLY

Background: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acety
Alias Symbols: ACL, ATPCL, CLATP
Protein Interaction Partner: UBC, UBR4, SIRT2, HUWE1, ISG15, KAT2B, SUMO2, SUMO3, STAU1, SUMO1, NEDD8, MDM2, ASB15, WWOX, TRIM25, MAGED2, BAG3, PPP6C, NCL, MCM6, MCM2, KPNA4, KPNA2, PRMT1, CDKN2A, SRRT, UBD, FN1, APP, SHC1, CUL3, CAND1, CUL2, SIRT7, UL27, HDAC5, TERF2, TERF1, GSK3B,
Protein Size: 1101

Molecular Weight: 121 kDa

Gene ID: 47

NCBI Accession: NM_001096, NP_001087

UniProt: P53396

Pathways: Warburg Effect