VASP antibody (Middle Region)

Catalog No. ABIN2774308

This Rabbit Polyclonal antibody specifically detects VASP in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Saccharomyces cerevisiae.

Quick Overview for VASP antibody (Middle Region) (ABIN2774308)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VASP Antibody

Binding Specificity: Middle Region

Sequence: WSVPNGPSPE EVEQQKRQQP GPSEHIERRV SNAGGPPAPP AGGPPPPPGP

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 90%, Human: 100%, Mouse: 91%, Rabbit: 92%, Rat: 92%, Yeast: 83%

Characteristics: This is a rabbit polyclonal antibody against VASP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human VASP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 380 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP

Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Alias Symbols: -
Protein Interaction Partner: ABI3, FGFR1OP, ABI2, SCGB2A2, CEP250, TP53, XIRP1, UBC, FAT1, FOXK1, PPP4R2, PAAF1, PRPF40A, RPP38, PRMT5, POP7, CUL4B, PSMA5, DDB1, APBB1, YWHAQ, NR4A1, YWHAE, DMRTB1, NPM1, VCAM1, SAT1, ITGA4, FN1, AMOT, SF3B2, SF3A3, ATP6V1F, CPNE3, SRSF9, UBE2N, UAP1,
Protein Size: 380

Molecular Weight: 42 kDa

Gene ID: 7408

NCBI Accession: NM_003370, NP_003361

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation