RNF31 antibody (Middle Region)

Catalog No. ABIN2774753

This anti-RNF31 antibody is a Rabbit Polyclonal antibody detecting RNF31 in WB. Suitable for Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse and Cow. This Primary Antibody has been cited in 8+ publications.

Quick Overview for RNF31 antibody (Middle Region) (ABIN2774753)

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF31 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF31 Antibody

Binding Specificity: Middle Region

Sequence: SLINAHSLDP ATLYEVEELE TATERYLHVR PQPLAGEDPP AYQARLLQKL

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF31. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF31

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1072 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RNF31 antibody (ABIN2774753)

Shibata, Tokunaga, Goto, Komatsu, Gohda, Saeki, Tanaka, Takahashi, Sawasaki, Inoue, Oshiumi, Seya, Nakano, Tanaka, Iwai, Inoue: "HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains." in: PLoS pathogens, Vol. 13, Issue 1, pp. e1006162, (2017) (PubMed).

Lafont, Kantari-Mimoun, Draber, De Miguel, Hartwig, Reichert, Kupka, Shimizu, Taraborrelli, Spit, Sprick, Walczak: "The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death." in: The EMBO journal, Vol. 36, Issue 9, pp. 1147-1166, (2017) (PubMed).

Kupka, De Miguel, Draber, Martino, Surinova, Rittinger, Walczak: "SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes." in: Cell reports, Vol. 16, Issue 9, pp. 2271-80, (2016) (PubMed).

Queisser, Dada, Deiss-Yehiely, Angulo, Zhou, Kouri, Knab, Liu, Stegh, DeCamp, Budinger, Chandel, Ciechanover, Iwai, Sznajder: "HOIL-1L functions as the PKCζ ubiquitin ligase to promote lung tumor growth." in: American journal of respiratory and critical care medicine, Vol. 190, Issue 6, pp. 688-98, (2014) (PubMed).

Blackwell, Zhang, Workman, Ting, Iwai, Habelhah: "Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate and delayed I?B kinase activation." in: Molecular and cellular biology, Vol. 33, Issue 10, pp. 1901-15, (2013) (PubMed).

Liu, Chen, Cai, Wu, Chen, Wu, Sun, Chen: "MAVS recruits multiple ubiquitin E3 ligases to activate antiviral signaling cascades." in: eLife, Vol. 2, pp. e00785, (2013) (PubMed).

Smit, Monteferrario, Noordermeer, van Dijk, van der Reijden, Sixma: "The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension." in: The EMBO journal, Vol. 31, Issue 19, pp. 3833-44, (2012) (PubMed).

Sieber, Lange, Kollmorgen, Erhardt, Quaas, Gontarewicz, Sass, Tiegs, Kreienkamp: "Sharpin contributes to TNF? dependent NF?B activation and anti-apoptotic signalling in hepatocytes." in: PLoS ONE, Vol. 7, Issue 1, pp. e29993, (2012) (PubMed).

Target Details for RNF31

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Alternative Name: RNF31

Background: PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.
Alias Symbols: FLJ10111, FLJ23501, MGC19975, ZIBRA, HOIP
Protein Interaction Partner: CCDC24, VASN, KRTAP9-2, TSSC4, UBC, SNRPC, PRKCA, AES, PITX1, KIF1A, SHARPIN, RBCK1, RNF31, MALT1, VCP, OTULIN, IRAK1, ESR1, Ikbkg, CCDC14, WRNIP1, AKAP8L, TRAF2, TNF, NDUFA8, BIRC2, NOD2, BIRC3, HCVgp1, SOCS3, SMAD3, NOS3, UBE2D3, UBE2D1, TNFAIP3, GFI1B,
Protein Size: 1072

Molecular Weight: 120 kDa

Gene ID: 55072

NCBI Accession: NM_017999, NP_060469

UniProt: Q96EP0