MID1 antibody (C-Term)

Catalog No. ABIN2775948

The Rabbit Polyclonal anti-MID1 antibody has been validated for WB. It is suitable to detect MID1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for MID1 antibody (C-Term) (ABIN2775948)

Target: MID1 (Midline 1 (MID1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MID1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MID1 Antibody

Binding Specificity: C-Term

Sequence: AINQAGSRSS EPGKLKTNSQ PFKLDPKSAH RKLKVSHDNL TVERDESSSK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MID1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MID1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 667 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MID1

Target: MID1 (Midline 1 (MID1))

Alternative Name: MID1

Background: MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing, however, the full length nature of two variants has not been determined.
Alias Symbols: OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, MIDIN, RNF59, ZNFXY, TRIM18
Protein Interaction Partner: STK36, MID2, UBE2E3, DYRK4, UBE2E2, UBE2E1, UBE2D3, UBE2D2, UBE2D1, PKN1, PPP2R4, PPP2CA, MID1, MEOX1, FKBP1A, EHHADH, BYSL, TCEANC, UBTD1, OTUB2, GMCL1, HTT, IGBP1, PNKP, MID1IP1, GNB2L1, RPS8, RPS3, NPM1, HSP90AA1, EEF1A1, ANXA2, UBC, UBE2D4, UBE2N, ELA
Protein Size: 667

Molecular Weight: 75 kDa

Gene ID: 4281

NCBI Accession: NM_000381, NP_000372

UniProt: O15344