NCS1 antibody (Middle Region)

Catalog No. ABIN2775968

This Rabbit Polyclonal antibody specifically detects NCS1 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Saccharomyces cerevisiaeand has been mentioned in 1 publication.

Quick Overview for NCS1 antibody (Middle Region) (ABIN2775968)

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NCS1 Antibody

Binding Specificity: Middle Region

Sequence: EEENTPEKRV DRIFAMMDKN ADGKLTLQEF QEGSKADPSI VQALSLYDGL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against FREQ. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FREQ

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 190 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NCS1 antibody (ABIN2775968)

Szafranski, Schindler, Taudien, Hiller, Huse, Jahn, Schreiber, Backofen, Platzer: "Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns." in: Genome biology, Vol. 8, Issue 8, pp. R154, (2008) (PubMed).

Target Details for NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Alternative Name: FREQ

Background: This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner
Alias Symbols: DKFZp761L1223, FLUP, NCS-1, NCS1, FREQ
Protein Interaction Partner: NBL1, DTX2, NAF1, KIF21A, KDM3B, SRP68, SNRPB, UBC, ELAVL1, PI4KB, KCND2, IL1RAPL1, DRD5, DRD3, DRD2, ADRBK1,
Protein Size: 190

Molecular Weight: 22 kDa

Gene ID: 23413

NCBI Accession: NM_014286, NP_055101

UniProt: P62166